ерeat ve 6 s. How many CFU/mL were in the original flask?

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 2.4P
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You culture some Listeria monocytogenes overnight in a flask. To determine their concentration, you take a 1 ml sample from the culture and add it
to 9 mL of PBS (buffered saline) (Tube 1). You take 1 mL from tube 1 and put it in 9 mL of PBS to make tube 2. You repeat this until you have 6
tubes. You take 0.1 mL from tube 6 and plate it on LB agar. The next day, you count 147 colonies. How many CFU/mL were in the original flask?
А.
1.47 x 105 CFU/mL
В.
1.47 x 10-5 CFU/mL
C.
1.47 x 103 CFU/mL
D.
1.47 x 10' CFU/mL
E.
1.47 x 108 CFU/mL
F.
1.47 x 10° CFU/mL
Transcribed Image Text:You culture some Listeria monocytogenes overnight in a flask. To determine their concentration, you take a 1 ml sample from the culture and add it to 9 mL of PBS (buffered saline) (Tube 1). You take 1 mL from tube 1 and put it in 9 mL of PBS to make tube 2. You repeat this until you have 6 tubes. You take 0.1 mL from tube 6 and plate it on LB agar. The next day, you count 147 colonies. How many CFU/mL were in the original flask? А. 1.47 x 105 CFU/mL В. 1.47 x 10-5 CFU/mL C. 1.47 x 103 CFU/mL D. 1.47 x 10' CFU/mL E. 1.47 x 108 CFU/mL F. 1.47 x 10° CFU/mL
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