Your lazy labmate needs your help! They set up a 10-ml culture of the slow-growing Myxococcus xanthus yesterday, but then they decided to stay up late playing videogames and won't be in until next Monday to recover. Whoops! They call you in lab that Friday and ask you to take an OD reading for them. You do this favor for them, and helpfully do the conversion into cells/mL, finding that their culture is currently sitting at about 3.0 x108 cells per mL. Your labmate is horrified: they won't be back in time before the cells hit stationary phase, since M. xanthus doubles every 5 hours. They beg you to make a new culture using these cells and set it up to reach the same cell density Monday morning. That's 59 hours away. If the cells usually lag for three hours upon dilution into fresh medium, what cell density should you dilute the culture to? About 1.3 x 107 cells/ml
Your lazy labmate needs your help! They set up a 10-ml culture of the slow-growing Myxococcus xanthus yesterday, but then they decided to stay up late playing videogames and won't be in until next Monday to recover. Whoops! They call you in lab that Friday and ask you to take an OD reading for them. You do this favor for them, and helpfully do the conversion into cells/mL, finding that their culture is currently sitting at about 3.0 x108 cells per mL. Your labmate is horrified: they won't be back in time before the cells hit stationary phase, since M. xanthus doubles every 5 hours. They beg you to make a new culture using these cells and set it up to reach the same cell density Monday morning. That's 59 hours away. If the cells usually lag for three hours upon dilution into fresh medium, what cell density should you dilute the culture to? About 1.3 x 107 cells/ml
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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