Ashu did a titer assay with her phage lysate. She started with 100µL of phage lysate (10) and did 7 1:10 dilutions down 107. She added 10ul of each dilution to 250µL of M. smeg and plated it on L-agar plates. Her 102 plate had 29 plaques. What is the titer of her lysate? O 290 pfu/ml.

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Ashu did a titer assay with her phage lysate. She started with 100μL of phage lysate (10%) and did 7 1:10 dilutions down to 10-7. She added 10μL of each dilution to 250μL of M. smeg and plated it on L-agar plates. Her 10-2 plate had 29 plaques. What is the titer of her lysate?
O 290 pfu/mL
O 2.9 X 106 pfu/mL
O 2900 pfu/mL
O 2.9 X 104 pfu/mL
O 2.9 X105 pfu/mL
Transcribed Image Text:Ashu did a titer assay with her phage lysate. She started with 100μL of phage lysate (10%) and did 7 1:10 dilutions down to 10-7. She added 10μL of each dilution to 250μL of M. smeg and plated it on L-agar plates. Her 10-2 plate had 29 plaques. What is the titer of her lysate? O 290 pfu/mL O 2.9 X 106 pfu/mL O 2900 pfu/mL O 2.9 X 104 pfu/mL O 2.9 X105 pfu/mL
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