Step #1 Culture and perform a Gram-stain on the Super Bug You culture the Super Bug by swabbing the patient's wound, applying the sample to the surface of an agar plate (petri dish), and incubating the plate for one day. Next you perform a Gram stain on a pure culture of the Super Bug cells. 1. Considering the bacteria were originally isolated from an exposed wound on a human patient, under what conditions (temperature, atmosphere) did you most likely incubate your plate of Super Bug cells to achieve maximal growth? Gram stain observations: Short rod-shaped cells found in clusters, stained red/pink 2. Is the Super Bug Gram negative or positive? 3. What conclusions can you make about the components of the Super Bug cell wall?

Step #1 Culture and perform a Gram-stain on the Super Bug You culture the Super Bug by swabbing the patient's wound, applying the sample to the surface of an agar plate (petri dish), and incubating the plate for one day. Next you perform a Gram stain on a pure culture of the Super Bug cells. 1. Considering the bacteria were originally isolated from an exposed wound on a human patient, under what conditions (temperature, atmosphere) did you most likely incubate your plate of Super Bug cells to achieve maximal growth? Gram stain observations: Short rod-shaped cells found in clusters, stained red/pink 2. Is the Super Bug Gram negative or positive? 3. What conclusions can you make about the components of the Super Bug cell wall?

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Sample Station 7 A. If a student performs a Gram stain on a Gram negative bacterium and stops the stain technique after the iodine step and rinse, what color would you predict the cells to be if you looked at it right now under the microscope?
1. The colonies on a negative MSA plate would appear _____________. 2. "E. Coli and S. epidermidis were chosen to represent Gram-negative and Gram-positive bacteria, respectively. For a given antibiotic, is there a difference in susceptibility between the Gram-positive and Gram-negative bacteria?" "No, they are usually similar" "yes, drugs that target the cell membrane are more effective on gram negatives" "yes, drugs that target the cell wall are more effective on gram negatives" "yes, drugs that target the cell wall are more effective on gram positives" how can I tell the difference
1- Why was the T4 and E.coli mixed together in top agar and then poured on the plate instead of just spreading the two together with a glass spreader on the plate? 2- When doing the titration and isolation of the T4 phage, E.coli diluted for you to 10e7 cells/ml. why was a diluted culture used? 3- After doing the titration of T4 phage, you find your dilution plates are all TNTC. how could this have happened? 4- Could you isolate phages that infect E.coli from raw sewerage? explain.
Put the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it. Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersion
In the image attached is a unknown bacteria lab. The gram stain result and growth result is already given to us and is shown. What other tests (1-2 examples) could we use to follow up this test. Explain why we should use those tests with justification.
For laboratory exercise of Antibiotic susceptibility test in disk-diffusion method. What does an ‘I’ result mean in disk diffusion susceptibility test?
Your first task in the lab is to learn how to culture these cells and begin expanding the culture (increasing the # of cells) so that you may later conduct experiments on them. You take two aliquots, each with an equal number of cells, and place them in two different culture vessels, one is a sealed airtight flask (culture 1) and the other is an open topped culture dish (culture 2). You provide both with the same growth media that contains their preferred fuel source, glucose. Are the cells in cultures 1 and 2 deriving all their energy in the same way? (Yes or No) Explain why or why not. Be sure to name the specific processes by which each culture is deriving its energy.
In order to do electron microscopy the samples had to be specially prepared. Were the cells alive at the time of viewing? Explain why you said yes or no I need help answering this queshtion the answer is with the article and it has to be as short as possible URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/