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Medium/test Gram stain TSA Eosin methylene blue agar (EMB) SIM motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red fermentation broth test media (list the specific carbs tested) phenylalanine agar urea agar or broth indicators, key used for/ determines reagents, or key positive result ingredients 1. crystal violet gram reaction and size and 2. iodine morfdant 3. gram's declorizer 4. safranin shape quadrant streak- isolation of pure culture of bacteria 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. 1. ferrous salts 2. Kovac's 3. low % agarose stains purple 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of medium/stab not visible negative result stains pink Comments/ additional info decolorization is key. Must include +/- controls on every slide to verify result check this for any pigmentation of colonies 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) in one medium) 3. stab is visible an defined with growth

Medium/test Gram stain TSA Eosin methylene blue agar (EMB) SIM motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red fermentation broth test media (list the specific carbs tested) phenylalanine agar urea agar or broth indicators, key used for/ determines reagents, or key positive result ingredients 1. crystal violet gram reaction and size and 2. iodine morfdant 3. gram's declorizer 4. safranin shape quadrant streak- isolation of pure culture of bacteria 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. 1. ferrous salts 2. Kovac's 3. low % agarose stains purple 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of medium/stab not visible negative result stains pink Comments/ additional info decolorization is key. Must include +/- controls on every slide to verify result check this for any pigmentation of colonies 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) in one medium) 3. stab is visible an defined with growth

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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indicators, key used for/ determines reagents, or key positive result ingredients 1. crystal violet Comments/ additional info Medium/test negative result decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result quadrant streak- isolation of pure culture of bacteria check this for any pigmentation of colonies TSA Eosin methylene blue agar (EMB) 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. ferrous salts 2. Kovac's SIM in one medium) 3. low % agarose medium/stab not visible motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red fermentation broth test media (list the specific carbs tested) phenylalanine agar urea agar or broth Methyl red test Vogues Proskauer test nitrate broth oxidase test casein agar gelatin deep lysine iron: lysine deaminase lysine decarboxylasse Pseudo. P agar Pseudo. F agar catalase test Mannitol Salt Agar Blood Agar with CAMP reaction Novobiocin sensitivity Bacitracin sensitivity bile esculin coagulase Lancefeild A Immunochromatograp hy test add any other additional tests here
Transcribed Image Text:indicators, key used for/ determines reagents, or key positive result ingredients 1. crystal violet Comments/ additional info Medium/test negative result decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result quadrant streak- isolation of pure culture of bacteria check this for any pigmentation of colonies TSA Eosin methylene blue agar (EMB) 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. ferrous salts 2. Kovac's SIM in one medium) 3. low % agarose medium/stab not visible motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red fermentation broth test media (list the specific carbs tested) phenylalanine agar urea agar or broth Methyl red test Vogues Proskauer test nitrate broth oxidase test casein agar gelatin deep lysine iron: lysine deaminase lysine decarboxylasse Pseudo. P agar Pseudo. F agar catalase test Mannitol Salt Agar Blood Agar with CAMP reaction Novobiocin sensitivity Bacitracin sensitivity bile esculin coagulase Lancefeild A Immunochromatograp hy test add any other additional tests here
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