Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
Help ASAP
Transcribed Image Text:6. Plasmid pBR322 includes two genes that confer antibiotic resistance one for ampicillin and one for tetracycline.
The cutting site for BamHI is in the middle of the tetracycline resistance gene. The cutting site for Pvul is in the
middle of the ampicillin resistance gene. You are planning to use tetracycline to isolate transformed bacteria.
Would you use the enzyme BamHI or the enzyme Pvul to cut the plasmid and excise the human gene you wish to
insert into it? Explair
7. Plasmids, which include the ampicillin resistance gene, are cut with a restriction enzyme that is also used to excise
a human gene from DNA sequences. The cut plasmids and the excised genes are mixed, annealed, and treated with
ligase. Bacteria are transformed with the plasmids using the calcium chloride method, and transformed bacteria are
selected using ampicillin. However, it proves, on investigation, that only some of the ampicillin resistant transformed
bacteria actually produce the protein coded for by the human gene; others fail to do so. Create a hypothesis to account
for this.
8. An individual is heterozygous for D1S80 and has the alleles of 23, 30. The frequency of 23 is 0.0119 while the
frequency of 30 is 0.0283. What is the genotypic frequency of this individual within the population
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education