Help ASAP 6. Plasmid pBR322 includes two genes that confer antibiotic resistance one for ampicillin and one for tetracycline.The cutting site for BamHI is in the middle of the tetracycline resistance gene. The cutting site for Pvul is in themiddle of the ampicillin resistance gene. You are planning to use tetracycline to isolate transformed bacteria.Would you use the enzyme BamHI or the enzyme Pvul to cut the plasmid and excise the human gene you wish toinsert into it? Explair7. Plasmids, which include the ampicillin resistance gene, are cut with a restriction enzyme that is also used to excisea human gene from DNA sequences. The cut plasmids and the excised genes are mixed, annealed, and treated withligase. Bacteria are transformed with the plasmids using the calcium chloride method, and transformed bacteria areselected using ampicillin. However, it proves, on investigation, that only some of the ampicillin resistant transformedbacteria actually produce the protein coded for by the human gene; others fail to do so. Create a hypothesis to accountfor this.8. An individual is heterozygous for D1S80 and has the alleles of 23, 30. The frequency of 23 is 0.0119 while thefrequency of 30 is 0.0283. What is the genotypic frequency of this individual within the population

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Plasm The c

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

Help ASAP

6. Plasmid pBR322 includes two genes that confer antibiotic resistance one for ampicillin and one for tetracycline.
The cutting site for BamHI is in the middle of the tetracycline resistance gene. The cutting site for Pvul is in the
middle of the ampicillin resistance gene. You are planning to use tetracycline to isolate transformed bacteria.
Would you use the enzyme BamHI or the enzyme Pvul to cut the plasmid and excise the human gene you wish to
insert into it? Explair
7. Plasmids, which include the ampicillin resistance gene, are cut with a restriction enzyme that is also used to excise
a human gene from DNA sequences. The cut plasmids and the excised genes are mixed, annealed, and treated with
ligase. Bacteria are transformed with the plasmids using the calcium chloride method, and transformed bacteria are
selected using ampicillin. However, it proves, on investigation, that only some of the ampicillin resistant transformed
bacteria actually produce the protein coded for by the human gene; others fail to do so. Create a hypothesis to account
for this.
8. An individual is heterozygous for D1S80 and has the alleles of 23, 30. The frequency of 23 is 0.0119 while the
frequency of 30 is 0.0283. What is the genotypic frequency of this individual within the population

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