The lacZ gene from E. coli encodes the enzyme B-galactosidase, which catalyzes conversion of a colorless compound called X-gal into a blue product. The lacZ gene is often used in plasmid vectors used for cloning. like the vector shown here. The amp' gene encodes an enzyme that makes a bacterial cell resistant to ampicillin. p CHELLA de pe care of HILLC lacz gene Origin of replication EcoRI site [Select] amp' If the plasmid shown in this figure is cut with EcoRI and then ligated with an EcoRI-digested fragment of human DNA, a bacterial colony containing the resulting recombinant DNA molecule will be [Select] and will be to ampicillin.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Pick the correct answer
1. White or blue
2. resistant or sensitive
![The lacZ gene from E. coli encodes the enzyme B-galactosidase, which
catalyzes conversion of a colorless compound called X-gal into a blue
product. The lacZ gene is often used in plasmid vectors used for cloning,
like the vector shown here.
The amp' gene encodes an enzyme that makes a bacterial cell resistant
to ampicillin.
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desabution without the prior written consent of McGraw Hill LLC.
lacz gene
Origin of
replication
EcoRI
site
[Select]
- amp'
If the plasmid shown in this figure is cut with EcoRI and then ligated with
an EcoRI-digested fragment of human DNA, a bacterial colony
containing the resulting recombinant DNA molecule will be
[Select]
and will be
to ampicillin.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F6ca1ab1e-fd0d-4a67-a37e-404a52e2e201%2F7746a509-19eb-44b6-8793-2caea46ded57%2F3f3qufr_processed.png&w=3840&q=75)
Recombinant DNA technology deals with changing the DNA sequence of the organisms. The molecular cloning is one such process that use plasmid (vector) to transfer a foreign DNA into the host organisms.
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