Consider the image below. This image shows plasmid DNA isolated through exactly the same method that you used. The lanes that were untreated (TE) contain plasmid DNA, but also large amounts of smaller nucleic acids that are predicted to be RNA. What is one explanation for why RNA could be present in the sample that should have contained plasmid DNA only? Think about the chemical similarities of these two molecules.

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Consider the image below. This image shows plasmid DNA isolated through exactly the same method that you used. The lanes that were untreated (TE) contain plasmid DNA, but also large amounts of smaller nucleic acids that are predicted to be RNA.

What is one explanation for why RNA could be present in the sample that should have contained plasmid DNA only? Think about the chemical similarities of these two molecules.

plasmid DNA
RNA
PUC18
pBR322
RNase TE DNase RNase DNase TE
1
2
3
5
6
location of blue dye front
Transcribed Image Text:plasmid DNA RNA PUC18 pBR322 RNase TE DNase RNase DNase TE 1 2 3 5 6 location of blue dye front
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Step 1

Plasmid DNA

In contrast to cell's chromosomal DNA, plasmidsare tiny, circular, double-stranded DNA molecules. 
Plasmids naturally exist in bacterial cells and certain eukaryotes as well.

 

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