constructing a map of the plasmid, pDiddy.   What is the smallest fragment size that the NcoI/EcoRI double digest produces?

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Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy.

 

What is the smallest fragment size that the NcoI/EcoRI double digest produces?

### Gel Electrophoresis Analysis

Gel electrophoresis is a technique used to separate DNA fragments based on their size. The image above depicts a gel electrophoresis result demonstrating the digestion of a DNA sample using various restriction enzymes.

**Marker Description:**

- **Lane L (Marker Lane):** This lane contains a DNA ladder used as a reference to estimate the size of the DNA fragments in the other lanes. The sizes of the markers in the DNA ladder are indicated on the left side of the gel: 3000 bp, 2000 bp, 1000 bp, 800 bp, 700 bp, and 500 bp.

**Sample Lanes and Enzyme Digestion:**

- **Uncut Lane:** Contains the undigested DNA sample. A prominent band appears around 3000 bp, indicating the total size of the uncut DNA fragment.

- **EcoRI Lane:** The DNA sample is digested with EcoRI enzyme. The digestion results in bands, notably one prominent band slightly below 3000 bp, indicating the cleavage of the DNA.

- **BamHI Lane:** The DNA sample is digested with BamHI enzyme. This digestion might result in different sized fragments, some of which are observed at distinct positions.

- **NcoI Lane:** The DNA sample is treated with NcoI enzyme, resulting in the formation of distinct DNA fragments that align at specific positions against the DNA ladder.

- **Combination Digestions:**
  - **EcoRI/BamHI:** DNA digested with both EcoRI and BamHI, resulting in multiple bands indicating DNA fragments of various sizes.
  - **BamHI/NcoI:** DNA sample digested with both BamHI and NcoI enzymes, resulting in the formation of distinct fragments.
  - **EcoRI/NcoI:** Simultaneous digestion with EcoRI and NcoI showing multiple bands.
  - **B/N/E (BamHI/NcoI/EcoRI):** Digestion with a combination of BamHI, NcoI, and EcoRI enzymes results in distinct bands that indicate multiple DNA fragments of specific sizes.

**Interpreting the Results:**

Analyzing the position and intensity of the bands in each lane allows for an understanding of the cutting efficiency and specificity of each enzyme or combination of enzymes. The bands are compared against the DNA ladder in Lane L to estimate the size of the fragments.

- DNA fragments located closer to the top of the gel
Transcribed Image Text:### Gel Electrophoresis Analysis Gel electrophoresis is a technique used to separate DNA fragments based on their size. The image above depicts a gel electrophoresis result demonstrating the digestion of a DNA sample using various restriction enzymes. **Marker Description:** - **Lane L (Marker Lane):** This lane contains a DNA ladder used as a reference to estimate the size of the DNA fragments in the other lanes. The sizes of the markers in the DNA ladder are indicated on the left side of the gel: 3000 bp, 2000 bp, 1000 bp, 800 bp, 700 bp, and 500 bp. **Sample Lanes and Enzyme Digestion:** - **Uncut Lane:** Contains the undigested DNA sample. A prominent band appears around 3000 bp, indicating the total size of the uncut DNA fragment. - **EcoRI Lane:** The DNA sample is digested with EcoRI enzyme. The digestion results in bands, notably one prominent band slightly below 3000 bp, indicating the cleavage of the DNA. - **BamHI Lane:** The DNA sample is digested with BamHI enzyme. This digestion might result in different sized fragments, some of which are observed at distinct positions. - **NcoI Lane:** The DNA sample is treated with NcoI enzyme, resulting in the formation of distinct DNA fragments that align at specific positions against the DNA ladder. - **Combination Digestions:** - **EcoRI/BamHI:** DNA digested with both EcoRI and BamHI, resulting in multiple bands indicating DNA fragments of various sizes. - **BamHI/NcoI:** DNA sample digested with both BamHI and NcoI enzymes, resulting in the formation of distinct fragments. - **EcoRI/NcoI:** Simultaneous digestion with EcoRI and NcoI showing multiple bands. - **B/N/E (BamHI/NcoI/EcoRI):** Digestion with a combination of BamHI, NcoI, and EcoRI enzymes results in distinct bands that indicate multiple DNA fragments of specific sizes. **Interpreting the Results:** Analyzing the position and intensity of the bands in each lane allows for an understanding of the cutting efficiency and specificity of each enzyme or combination of enzymes. The bands are compared against the DNA ladder in Lane L to estimate the size of the fragments. - DNA fragments located closer to the top of the gel
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