How many DNA fragments will result when the double-stranded DNA molecule below is digested with HindIII? 5'-AAGAATTAAGCTTATTCGGAAGCTTAGCGCCGCGAAGCTTCCTTAAA-3' 3'-TTCTTAATTCGAATAAGCCTTCGAATCGCGGCGCTTCGAAGGAATTT-5' HindIll target sequence: 5'-A^AGCTT-3' 3'-TTCGA^A-5' Three
How many DNA fragments will result when the double-stranded DNA molecule below is digested with HindIII? 5'-AAGAATTAAGCTTATTCGGAAGCTTAGCGCCGCGAAGCTTCCTTAAA-3' 3'-TTCTTAATTCGAATAAGCCTTCGAATCGCGGCGCTTCGAAGGAATTT-5' HindIll target sequence: 5'-A^AGCTT-3' 3'-TTCGA^A-5' Three
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:How many DNA fragments will result when the double-stranded DNA
molecule below is digested with Hindill?
5'-AAGAATTAAGCTTATTCGGAAGCTTAGCGCCGCGAAGCTTCCTTAAA-3′
3'-TTCTTAATTCGAATAAGCCTTCGAATCGCGGCGCTTCGAAGGAATTT-5'
HindIll target sequence:
5'-A^AGCTT-3'
3'-TTCGA^A-5'
Three
Two
Four
One
Transcribed Image Text:The following diagram shows a plasmid (vector) backbone in which you want to clone the coding
sequence (cds) of a particular protein to overexpress it. The restriction enzymes that cut this
plasmid are also represented. Which restriction enzymes would you use to open the plasmid and
clone your sequence of interest?
Promoter
HindlII+
Xhol +
Polyadenylation signal
O Hindill or Xhol
EcoRV
O EcoRV, Kpnl or EcoRI
O Notl or BamH1
Kpnl
A
EcoRI
Oric
Kanamycin
resistance
O This plasmid cannot be used to overexpress a protein because it lacks several key features to carry this
function
→ Notl
→ BamHI
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