Agarose gel Can someone help me to understand how to estimate the size of my plasmid cut (pla cut) and insert (PCR cut) that shows in the picture? thank you (its from an cloning experiment)
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Agarose gel
Can someone help me to understand how to estimate the size of my plasmid cut (pla cut) and insert (PCR cut) that shows in the picture?
thank you
(its from an cloning experiment)
![bp ng/0.5 µg %
20000 20 4
10000 20 4
7000 20 4
5000 75 15
4000 20 4
3000 20 4
2000 20 4
1500 80 16
1000 25 5
700 25 5
500 75 15
400
25 5
300
200
75
25 5
25
25
0.5 µg/lane, 8 cm length gel,
1X TAE, 7 V/cm, 45 min
MW Pla Pla PCR PCR
marker cut uncut cut uncut
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