You use BamHI to cut open an empty plasmid vector and insert a gene. The following charts show the restriction digests of the empty plasmid and the plasmid containing the inserted gene. Use them to answer the questions below: Empty Plasmid BamHI 9.0Kb EcoRV 6.0Kb 3.0Kb BamHI + EcoRV 6.0Kb 2.0Kb |1.0Kb Plasmid with Insert BamHI 9.0Kb |1.ОКЬ EcoRV 6.0Kb 2.2Kb 1.8Kb What is the size of the empty plasmid? 9.0Kb What is the size of the insert? 1.0 KЬ From smallest to largest, what sizes of fragments would be created by digestion of the plasmid with insert by BamHI and Hindll1?
You use BamHI to cut open an empty plasmid vector and insert a gene. The following charts show the restriction digests of the empty plasmid and the plasmid containing the inserted gene. Use them to answer the questions below: Empty Plasmid BamHI 9.0Kb EcoRV 6.0Kb 3.0Kb BamHI + EcoRV 6.0Kb 2.0Kb |1.0Kb Plasmid with Insert BamHI 9.0Kb |1.ОКЬ EcoRV 6.0Kb 2.2Kb 1.8Kb What is the size of the empty plasmid? 9.0Kb What is the size of the insert? 1.0 KЬ From smallest to largest, what sizes of fragments would be created by digestion of the plasmid with insert by BamHI and Hindll1?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:You use BamHI to cut open an empty plasmid vector and insert a gene.
The following charts show the restriction digests of the empty plasmid and the
plasmid containing the inserted gene. Use them to answer the questions below:
Empty Plasmid
BamHI
9.0Kb
EcoRV
6.0Kb
3.0Kb
BamHI + EcoRV
6.0Kb
2.0Kb
|1.0Kb
Plasmid with Insert
BamHI
9.0Kb
|1.ОКЬ
EcoRV
6.0Kb
2.2Kb
1.8Kb
What is the size of the empty plasmid?
9.0Kb
What is the size of the insert?
1.0 KЬ
From smallest to largest, what sizes of fragments would be created by digestion of
the plasmid with insert by BamHI and Hindll1?
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