A eukaryotic gene is added to a mixture containing a restriction enzyme EcoRI and a bacterial plasmid that carrie genes conferring resistance to ampicillin and tetracycline. The plasmid has a recognition site for EcoRI located ir tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then adder bacteria growing in nutrient broth. The bacteria are allowed to grow overnight on a plate using a technique t produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin nutrient broth plus tetracycline nutrient broth plus ampicillin and tetracycline the nutrient broth only (without antibiotics) ● Question: Which of the following media would allow the growth of bacteria containing a plasmid with a eukaryotic gene? the nutrient broth only the nutrient broth and the ampicillin broth only ● the nutrient broth and the tetracycline broth only the nutrient broth, the ampicillin broth, and the tetracycline broth. ●

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Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.