sticky enas DNA strands have the same base-pair sequence when read in opposite directions. farther from Some restriction enzymes cut both strands at the line of symmetry in the middle of the recognition sequence. These enzymes generate bases closer to Other enzymes cut the two strands at sites that are offset from the center in opposite directions. These enzymes result in short single strands called covalent bonds If the four bases are distributed randomly and present in equal proportions, the fragments size produced by a restriction enzyme is 47, where n is the number of bases in the An enzyme that recognizes a specific four-base sequence generates fragments averaging bp in length, whereas one that recognizes a six-base sequence generates fragments averaging genome bp in length. average length of by the average The number of fragments produced can be estimated by dividing the length of fragments produced by the enzyme. 256 number of bases in the recognition sequence Gel electrophoresis can separate DNA fragments from a restriction enzyme digest by Small fragments are the wells than large fragments. palindromic charge Reset 4096 blunt ends A restriction enzyme recognizes a specific sequence of anywhere within a genome and (one in each strand) at particular positions within or near that sequence. then severs two longest The recognition sites for most restriction enzymes consist of DNA strands have the same base-pair sequence when read in opposite directions. sequences in which the two sticky ends farther from Some restriction enzymes cut both strands at the line of symmetry in the middle of the recognition sequence. These enzymes generate bases closer to Other enzymes cut the two strands at sites that are offset from the center in opposite directions. These enzymes result in short single strands called covalent bonds If the four bases are distributed randomly and present in equal proportions, the fragments size produced by a restriction enzyme is 4", where n is the number of bases in the An enzyme that recognizes a specific four-base sequence generates fragments averaging bp in length, whereas one that recognizes a six-base sequence generates fragments averaging genome bp in length. average length of by the average The number of fragments produced can be estimated by dividing the length of fragments produced by the enzyme. 256 number of bases in the recognition sequence Gel electrophoresis can separate DNA fragments from a restriction enzyme digest by Small fragments are the wells than large fragments. palindromic