lili マ四 Format Tools Table Window Help Indigraons Injustice in HealthCare w Forbes 8am g - Saved Layout References Mailings Review View Tell me A A Aa v AaBbCcDdEe AaBbCcDdEe Styles Pane Normal No Spacing x, x A X. 田,|^三 = === Tube 2-U.TML mIK iromITUve T uluted in 9.9 ML stenie water Tube 3 – 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water What is the dilution of raw milk in Tube 3? 00001 2. A researcher analyzes unpasteurized apple juice suspecting E. coli contamination of the juice. He/she plates out 0.1 mL of a 10-2 dilution of juice on each plate and grows up the plates. He/she then counts colonies on each plate. Plate 1 – 242 colonies Plate 2 – 237 colonies Plate 3 – 249 colonies (a) Calculate the CFU/mL for this juice. Show formula used and include units. 王 (b) If acceptable levels of E. coli in juice were 0.02 CFU/ml juice, would this juice be acceptable for human consumption? Why or why not? (3) 3. There is concern that some fresh strawberries were contaminated with Listeria monocytogenes. Samples of the strawberries were taken and analyzed. The researcher collected the samples and then plated out 0.1ml/plate of ground strawberries diluted to 1/1000X. After time for growth the plates had the nglish (United States) NOV 62 étv

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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lili
マ四
Format
Tools
Table
Window
Help
Indigraons
Injustice in
HealthCare
w Forbes 8am g - Saved
Layout
References
Mailings
Review
View Tell me
A A Aa v
AaBbCcDdEe
AaBbCcDdEe
Styles
Pane
Normal
No Spacing
x, x A
X.
田,|^三 = ===
Tube 2-U.TML mIK iromITUve T uluted in 9.9 ML stenie water
Tube 3 – 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water
What is the dilution of raw milk in Tube 3?
00001
2. A researcher analyzes unpasteurized apple juice suspecting E. coli
contamination of the juice. He/she plates out 0.1 mL of a 10-2 dilution of juice on
each plate and grows up the plates. He/she then counts colonies on each plate.
Plate 1 – 242 colonies
Plate 2 – 237 colonies
Plate 3 – 249 colonies
(a) Calculate the CFU/mL for this juice. Show formula used and include units.
王
(b) If acceptable levels of E. coli in juice were 0.02 CFU/ml juice, would this juice
be acceptable for human consumption?
Why or why not?
(3) 3. There is concern that some fresh strawberries were contaminated with
Listeria monocytogenes. Samples of the strawberries were taken and analyzed.
The researcher collected the samples and then plated out 0.1ml/plate of ground
strawberries diluted to 1/1000X. After time for growth the plates had the
nglish (United States)
NOV 62
étv
Transcribed Image Text:lili マ四 Format Tools Table Window Help Indigraons Injustice in HealthCare w Forbes 8am g - Saved Layout References Mailings Review View Tell me A A Aa v AaBbCcDdEe AaBbCcDdEe Styles Pane Normal No Spacing x, x A X. 田,|^三 = === Tube 2-U.TML mIK iromITUve T uluted in 9.9 ML stenie water Tube 3 – 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water What is the dilution of raw milk in Tube 3? 00001 2. A researcher analyzes unpasteurized apple juice suspecting E. coli contamination of the juice. He/she plates out 0.1 mL of a 10-2 dilution of juice on each plate and grows up the plates. He/she then counts colonies on each plate. Plate 1 – 242 colonies Plate 2 – 237 colonies Plate 3 – 249 colonies (a) Calculate the CFU/mL for this juice. Show formula used and include units. 王 (b) If acceptable levels of E. coli in juice were 0.02 CFU/ml juice, would this juice be acceptable for human consumption? Why or why not? (3) 3. There is concern that some fresh strawberries were contaminated with Listeria monocytogenes. Samples of the strawberries were taken and analyzed. The researcher collected the samples and then plated out 0.1ml/plate of ground strawberries diluted to 1/1000X. After time for growth the plates had the nglish (United States) NOV 62 étv
Expert Solution
Step 1

A colony-forming unit (CFU) is a unit that is used in microbiology to measure the viable number of bacteria or fungal cells in a particular sample. Viable means the capability to multiply and divide under controlled conditions. The visual appearance of bacteria or fungal cells as a colony in cell culture requires significant growth. For liquid mediums, the results are given in CFU/ml (colony-forming units per milliliter) whereas, for solids, it is measured in CFU/g (colony-forming units per gram)

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