Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. F the EcoRI/BamHI double digest produces 3 fragments with only two sizes, what are their sizes?     2.5 kb, 250 b and 250 b     3.0 kb     2.0 kb 500 b and 500 b     3.0 kb 1.0kb and 1.0 kb

Q: Eco01091 2074 Pfol 46 BstAPI 170 Aatil 2017 Sspl 2501 Ndel 183 Ehel 235 Pdml 2204, Bogi 2215 Soal…

A: Restriction endonucleases also referred to as restriction enzymes are enzymes that cut DNA at…

Q: Which would you choose if you needed to make a partial diploid for complementation analysis? rolling…

A: The correct answer is option 1. Rolling circle plasmid.

Q: In the genotype presented (genomic plus plasmid genes), which of the following statements Is…

A: Lac operon codes Lac Z,Y,A genes which are structural genes encoding Beta galactosidase,Permease,…

Q: A.  Draw a map of the F+strain, indicating the positions of all genes and their distances apart in…

A:

Q: Provide 2 diagrams to illustrate the 2 potential recombinant plasmids,

A: The question here states about the concerned recombination of cloned plasmids and their…

Q: Plasmid is  3.6kb large. It is digested with an enzyme that has restriction sites at  1,152 and…

A: Plasmids are circular DNA found in bacteria. They are extra chromosomal materials and are highly…

Q: Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the…

A: The next stage is called "isolation of target DNA," and it involves removing the DNA that contains…

Q: etion enzyme Sall cuts the sequence GTCGAC to leave a five base, 5' overhang. The restriction enzyme…

A: A restriction enzyme is classified under the category of a protein. These are isolated from bacteria…

Q: Which of the following statements are TRUE? (Multiple Select - choose ALL that are TRUE) O A…

A: Bacteria is the unicellular organisms they are different types of bacteria many lives in different…

Q: List the major steps that would be required to clone the human insulin gene into a plasmid and to…

A: Gene cloning is the isolation of DNA (deoxyribonucleic acid) sequence or gene of interest and…

Q: The following plasmid is digested with EcoR1 and Ndel restriction endonucleases. Agarose…

A: Restriction endonucleases are enzymes which cleave the phosphodiester bonds within a DNA. These…

Q: 1 2 3 4 5 6 7 8 9 10,000 6,0008,00 5,000 4,000 3,000 2,500 2,000 1,500 1. 1kb DNA ladder 2. G9-…

A: Based on their size, the DNA fragments are separated and analyzed in a gel electrophoresis system. A…

Q: This is a map of PBR322 plasmid Nid If a recombinant plasmid were obtained inserting DNA into the…

A: Ans: Recombinant DNA technology: The technique of cutting and pasting of DNA molecule with another…

Q: Using a diagram, and 3-5 sentences, explain the relationship between cell immortality and telomerase…

A: Each time a cell divides, the telomeres get shorter. Telomeres are a region of repetitive nucleotide…

Q: Consider the image below. This image shows plasmid DNA isolated through exactly the same method that…

A: Plasmid DNA In contrast to a cell's chromosomal DNA, plasmidsare tiny, circular, double-stranded DNA…

Q: Agarose gel Can someone help me to understand how to estimate the size of my plasmid cut (pla cut)…

A: The procedure of inserting recombinant DNA into a vector, or a carrier of DNA molecules, to…

Q: Can you answer the last three q

A: Note: As per Bartleby Guidelines For Remaining Answers Please Repost The Question. Introduction:…

Q: What color colonies will cells that contain a recombinant plasmid form?

A: Recombinant plasmids are the vectors that carry target DNA or gene sequence to the host by the help…

Q: After transformation you were asked to grow bacterial cells transformed with plasmid on a plate that…

A: Bacterial transformation is a process through which bacteria can take up foreign DNA. Transformation…

Q: С 17 Based on the knowledge you gained from the cloning module, which of the bands in the figure is…

A: Agarose gel electrophoresis is a method used in molecular biology to resolve DNA fragments on the…

Q: A student wanted to load .75 ug dna on agarose gel and has 4x loading buffer for sample preparation.…

A: Agarose gel electrophoresis is a method used for the separation of DNA fragments which is stained by…

Q: ori Clo fcoR v OFP Below is the plasmid map of pGLO which contains the Green Fluorescent Protein…

A: pGLO:   pGLO is an engineered plasmid that contains genes for beta- Lactamase.  Beta-lactamase…

Q: Assume the samples in this diagram of gel electrophoresis are DNA molecules. Why do the samples…

A: Gel electrophoresis is used to separate molecules of different sizes. It uses charged molecules that…

Q: Which of the following would allow for control of a gene's orientation when inserted into a plasmid…

A: The technique of molecular biology used to generate similar copies of a particular segment (gene) of…

Q: dont provide handwriting solution ...

A: When dealing with circular DNA containing multiple EcoRI recognition sites, the digestion pattern…

Q: F plasmid F’ plasmid (and how is it formed) Hfr cell (and how is it formed)

A: Plasmids are the extrachromosomal material that exist apart from the main chromosome in a bacterial…

Q: Give typed full explanation  you made a recombinant plasmid (it is circular) that contains the…

A: Here, the correct answer is option 1) The plasmid will be cut once, making a linear DNA fragment.

Q: Summary table of data for one yeast DNA microarray. Shows 14 of the 6,200 genes on the full…

A: A DNA microarray also known as a DNA chip or gene chip is a high throughput technology used to study…

Q: How many DNA fragments will result when the double-stranded DNA molecule below is digested with…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Write the process of, building a recombinant plasmid? Or how you can build a recombinant plasmid?

A: A plasmid is a circular, single-stranded DNA molecule that is distinct from a cell's chromosomal…

Q: Uncut - DNA Fragments produced B2 Sspl Enzyme(s) EcoR1 Smal used - 5.0 plasmid, how many times does…

A: Introduction :  The DNA-based structure known as a plasmid can exist and replicate on its own in…

Q: A plasmid comprised of B-DNA changed its linking number from 420 to 457, resulting in a superhelix…

A: Introduction: DNA in the cell must be arranged in order to allow the packing of large DNA molecules…