Answered: You utilised two plasmids in this…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

You utilised two plasmids in this practical, pOTC and pOTC-Δ. Plasmids are often represented using plasmid maps like the one below. This map shows the positions of recognition sites for a number of restriction enzymes

Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if this plasmid was digested with restriction enzymes EcoRI and PvuII.

Pvull
(6103)
Pstl
(6789)
Bstl
(4469)
pBCH2.0
(7693 bp)
EcoRV
(623)
Xbal
(3188)
Xbal
(1282)
EcoRI (1538)
BamHI (1545)
Kpnl (1613)
Hindill (1653)
Sall
(1691)
Pstl
(2401)

Expert Solution

Step 1

Bacteria produce the restriction enzyme, a protein that cleaves DNA at certain locations. The "restriction site" is this site. A crucial component of genetic engineering is restriction enzymes. Restriction enzymes are key genetic engineering tools. They are extracted from bacteria and utilized in laboratory experiments. Recognition sequences are short and particular nucleotide sequences in DNA that restriction enzymes recognize. When a restriction enzyme detects a DNA sequence, it hydrolyzes the link between nearby nucleotides and breaks the DNA molecule.