Use the plasmid map for pBashi below to answer the following questions Hind!!! 4 kb Xhol 1 kb Xhol O 3 kb 7 kb Hindill a. How large is pBashi? b. On the gel below, draw the bands corresponding to the expected pBashi cleavage products after restriction digest with the indicated restriction enzymes (Lane 2 - Xhol only, Lane 3 Hindill only, Lane 4 - Xhol+ HindIII) 15 kb 11 kb 10 kb 8 kb 7 kb 5 kb 4 kb 3 kb 2 kb 1 kb Xhol Xhol Xhol Hindill Pstl Pstl Ladder Xhol Hind!!! Hind!!! ||| c. Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ Pstl. Lane 6 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ HindlII+ Pstl. Indicate your final answers to the questions below clearly on the pBashi plasmid above; you will NOT receive any credit if you only list sizes of fragments below. If you need to, draft your answer somewhere else before writing your final answer above. i) The location of the Pstl site ii) The sizes of the new fragments made by this Pstl cut
Use the plasmid map for pBashi below to answer the following questions Hind!!! 4 kb Xhol 1 kb Xhol O 3 kb 7 kb Hindill a. How large is pBashi? b. On the gel below, draw the bands corresponding to the expected pBashi cleavage products after restriction digest with the indicated restriction enzymes (Lane 2 - Xhol only, Lane 3 Hindill only, Lane 4 - Xhol+ HindIII) 15 kb 11 kb 10 kb 8 kb 7 kb 5 kb 4 kb 3 kb 2 kb 1 kb Xhol Xhol Xhol Hindill Pstl Pstl Ladder Xhol Hind!!! Hind!!! ||| c. Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ Pstl. Lane 6 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ HindlII+ Pstl. Indicate your final answers to the questions below clearly on the pBashi plasmid above; you will NOT receive any credit if you only list sizes of fragments below. If you need to, draft your answer somewhere else before writing your final answer above. i) The location of the Pstl site ii) The sizes of the new fragments made by this Pstl cut
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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