Use the plasmid map for pBashi below to answer the following questions Hind!!! 4 kb Xhol 1 kb Xhol O 3 kb 7 kb Hindill a. How large is pBashi? b. On the gel below, draw the bands corresponding to the expected pBashi cleavage products after restriction digest with the indicated restriction enzymes (Lane 2 - Xhol only, Lane 3 Hindill only, Lane 4 - Xhol+ HindIII) 15 kb 11 kb 10 kb 8 kb 7 kb 5 kb 4 kb 3 kb 2 kb 1 kb Xhol Xhol Xhol Hindill Pstl Pstl Ladder Xhol Hind!!! Hind!!! ||| c. Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ Pstl. Lane 6 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ HindlII+ Pstl. Indicate your final answers to the questions below clearly on the pBashi plasmid above; you will NOT receive any credit if you only list sizes of fragments below. If you need to, draft your answer somewhere else before writing your final answer above. i) The location of the Pstl site ii) The sizes of the new fragments made by this Pstl cut

Human Anatomy & Physiology (11th Edition)
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Use the plasmid map for pBashi below to answer the following questions
Xhol
Hind!!!
15 kb
11 kb
10 kb
8 kb
7 kb
5 kb
a. How large is pBashi?
b. On the gel below, draw the bands corresponding to the expected pBashi cleavage products after restriction digest with the indicated restriction enzymes (Lane 2 - Xhol only, Lane 3 -
HindIII only, Lane 4 - Xhol+ HindIII)
4 kb
3 kb
3 kb
2 kb
1 kb
4 kb
Hindill
1 kb
7 kb
Xhol
Xhol
Xhol Xhol
Hind!!!
Ladder Xhol Hindill Hind!!! Pstl Pstl
c. Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ Pstl. Lane 6 in the gel above displays the pBashi cleavage
products after restriction digest with the restriction enzymes Xhol+ HindIII + Pstl. Indicate your final answers to the questions below clearly on the pBashi plasmid above; you will NOT
receive any credit if you only list sizes of fragments below. If you need to, draft your answer somewhere else before writing your final answer above.
i) The location of the Pstl site
ii) The sizes of the new fragments made by this Pstl cut
Transcribed Image Text:Use the plasmid map for pBashi below to answer the following questions Xhol Hind!!! 15 kb 11 kb 10 kb 8 kb 7 kb 5 kb a. How large is pBashi? b. On the gel below, draw the bands corresponding to the expected pBashi cleavage products after restriction digest with the indicated restriction enzymes (Lane 2 - Xhol only, Lane 3 - HindIII only, Lane 4 - Xhol+ HindIII) 4 kb 3 kb 3 kb 2 kb 1 kb 4 kb Hindill 1 kb 7 kb Xhol Xhol Xhol Xhol Hind!!! Ladder Xhol Hindill Hind!!! Pstl Pstl c. Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ Pstl. Lane 6 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ HindIII + Pstl. Indicate your final answers to the questions below clearly on the pBashi plasmid above; you will NOT receive any credit if you only list sizes of fragments below. If you need to, draft your answer somewhere else before writing your final answer above. i) The location of the Pstl site ii) The sizes of the new fragments made by this Pstl cut
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