Use the plasmid map for pBashi below to answer the following questions Hind!!! 4 kb Xhol 1 kb Xhol O 3 kb 7 kb Hindill a. How large is pBashi? b. On the gel below, draw the bands corresponding to the expected pBashi cleavage products after restriction digest with the indicated restriction enzymes (Lane 2 - Xhol only, Lane 3 Hindill only, Lane 4 - Xhol+ HindIII) 15 kb 11 kb 10 kb 8 kb 7 kb 5 kb 4 kb 3 kb 2 kb 1 kb Xhol Xhol Xhol Hindill Pstl Pstl Ladder Xhol Hind!!! Hind!!! ||| c. Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ Pstl. Lane 6 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ HindlII+ Pstl. Indicate your final answers to the questions below clearly on the pBashi plasmid above; you will NOT receive any credit if you only list sizes of fragments below. If you need to, draft your answer somewhere else before writing your final answer above. i) The location of the Pstl site ii) The sizes of the new fragments made by this Pstl cut

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Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.