B. Arrange the following steps in the genetic engineering of corn to acquire pest resistance. to indicate the correct sequence. The tumor-inducing plasmid will be incorporated into the genome of the infected corn cells. Write numbers 1 to 1O 1. 2. Insert the cry1Ab gene into the tumor-inducing plasmid of Agrobacterium 3. tumefaciens by using ligase. Treat the isolated genetic material with restriction enzymes and subject it to gel electrophoresis and probes to identify and isolate the cry1Ab gene. 4. Regenerate the cultured corn cells and allow them to grow into mature Bt corn. 5. Reintroduce the recombinant plasmid into Agrobacterium tumefaciens cells. 6. Allow the transformed Agrobacterium tumefaciens cells to infect the cultured corn cells. 7. Cross the mature Bt corn with other superior corn varieties. 8. Obtain and culture Bacillus thuringiensis cells as sources of the cry1Ab gene. 9. Determine the source of the gene of interest which expresses the pest resistance trait. 10. Isolate the genetic material from Bacillus thuringiensis. C. Answer the following question. 1. If you are able to successfully incorporate foreign DNA to your host organism, whatare your major concerns thereafter? 9 | MODULE NUMBER 1 THIRD QUARTER
B. Arrange the following steps in the genetic engineering of corn to acquire pest resistance. to indicate the correct sequence. The tumor-inducing plasmid will be incorporated into the genome of the infected corn cells. Write numbers 1 to 1O 1. 2. Insert the cry1Ab gene into the tumor-inducing plasmid of Agrobacterium 3. tumefaciens by using ligase. Treat the isolated genetic material with restriction enzymes and subject it to gel electrophoresis and probes to identify and isolate the cry1Ab gene. 4. Regenerate the cultured corn cells and allow them to grow into mature Bt corn. 5. Reintroduce the recombinant plasmid into Agrobacterium tumefaciens cells. 6. Allow the transformed Agrobacterium tumefaciens cells to infect the cultured corn cells. 7. Cross the mature Bt corn with other superior corn varieties. 8. Obtain and culture Bacillus thuringiensis cells as sources of the cry1Ab gene. 9. Determine the source of the gene of interest which expresses the pest resistance trait. 10. Isolate the genetic material from Bacillus thuringiensis. C. Answer the following question. 1. If you are able to successfully incorporate foreign DNA to your host organism, whatare your major concerns thereafter? 9 | MODULE NUMBER 1 THIRD QUARTER
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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B. Arrange the following
C. Answer the question
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Transcribed Image Text:B. Arrange the following steps in the genetic engineering of corn to acquire pest resistance.
to indicate the correct sequence.
The tumor-inducing plasmid will be incorporated into the genome of the infected corn cells.
Write numbers 1 to 1O
1.
2.
Insert the cry1Ab gene into the tumor-inducing plasmid of Agrobacterium
3.
tumefaciens by using ligase. Treat the isolated genetic material with restriction enzymes and
subject it to gel electrophoresis and probes to identify and isolate the cry1Ab gene.
4.
Regenerate the cultured corn cells and allow them to grow into mature Bt corn.
5.
Reintroduce the recombinant plasmid into Agrobacterium tumefaciens cells.
6.
Allow the transformed Agrobacterium tumefaciens cells to infect the cultured corn cells.
7.
Cross the mature Bt corn with other superior corn varieties.
8.
Obtain and culture Bacillus thuringiensis cells as sources of the cry1Ab gene.
9.
Determine the source of the gene of interest which expresses the pest resistance trait.
10. Isolate the genetic material from Bacillus thuringiensis.
C. Answer the following question.
1. If you are able to successfully incorporate foreign DNA to your host organism, whatare your
major concerns thereafter?
9 | MODULE NUMBER 1 THIRD QUARTER
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