ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it nonfunctional, how would that affect the level of B-galactosidase protein activity with and without lactose present, why?
ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it nonfunctional, how would that affect the level of B-galactosidase protein activity with and without lactose present, why?
Chapter16: Adult And Pediatric Dosages Based On Body Surface Area
Section: Chapter Questions
Problem 7.2P
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Question
Part ii. ) please
![i.) Fill in the table for each of the E. coli: (0) = No Activity (+)= Basal Activity and (+++) = High Activity
E. coli chromosome
F Plasmid
B-gal activity?
Permease activity?
When
Lactose is
present
When
Lactose is
present
When
When
Glucose is
present
Glucose is
present
+++
+++
a.) I+ P+ O+ Z+ Y+
none
b.) I^[S] P+ O+ Z+ Y+
none
10
0.
c.) I+ P+ O^[c] Z+ Y+
none
+++
+++
+++
+++
d.) I+ P+ O- Z- Y+
0.
none
+++
+++
e.) I+ P+ O+ Z+ Y+
I^[S] P+ O+ Z+ Y+
f.) I[S] P+ O+ Z- Y+
+ P+ O^[c] Z+ Y-
9.) I^[TB] P+ O+ Z+ Y
I+ P+ O^[c] Z- Y+
h.) I+ P+ O^[c] Z+ Y-
I+ P+ O+ Z** Y+
i.) I^[TB] P+ O^[c] Z+ Y- 1+ P+ O+ Z- Y+
Z** is a polar mutation
ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it
nonfunctional, how would that affect the level of B-galactosidase protein activity with
and without lactose present, why?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F489ac520-fb4a-49de-9034-10284a50d879%2Fab02a38a-8247-492d-83f5-ce360876e11a%2Fwwl9ulz_processed.jpeg&w=3840&q=75)
Transcribed Image Text:i.) Fill in the table for each of the E. coli: (0) = No Activity (+)= Basal Activity and (+++) = High Activity
E. coli chromosome
F Plasmid
B-gal activity?
Permease activity?
When
Lactose is
present
When
Lactose is
present
When
When
Glucose is
present
Glucose is
present
+++
+++
a.) I+ P+ O+ Z+ Y+
none
b.) I^[S] P+ O+ Z+ Y+
none
10
0.
c.) I+ P+ O^[c] Z+ Y+
none
+++
+++
+++
+++
d.) I+ P+ O- Z- Y+
0.
none
+++
+++
e.) I+ P+ O+ Z+ Y+
I^[S] P+ O+ Z+ Y+
f.) I[S] P+ O+ Z- Y+
+ P+ O^[c] Z+ Y-
9.) I^[TB] P+ O+ Z+ Y
I+ P+ O^[c] Z- Y+
h.) I+ P+ O^[c] Z+ Y-
I+ P+ O+ Z** Y+
i.) I^[TB] P+ O^[c] Z+ Y- 1+ P+ O+ Z- Y+
Z** is a polar mutation
ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it
nonfunctional, how would that affect the level of B-galactosidase protein activity with
and without lactose present, why?
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