ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it nonfunctional, how would that affect the level of B-galactosidase protein activity with and without lactose present, why?
Q: Which of the following is the correct sequence for the aseptic transfer of a bacterial culture?…
A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…
Q: A client is admitted to the hospital with deep vein thrombosis. As infusion of heparin is…
A: Deep vein thrombosis is vascular clotting disorder in which there is blood clots in the deep vein…
Q: What are the financial incentives and penalties of HIPPA requirement for third-party payers
A: The objective of this question is to understand the financial incentives and penalties associated…
Q: QUESTION 33 The primary xenobiotic degrading genus are: O a. Staphlococcus O b. Streptococcus O c.…
A: Plant elements, pharmaceuticals, pesticides, cosmetics, flavourings, scents, food additives,…
Q: Avoiding the use of tanning parlors or sunlamps is one of the best ways to ____. a. limit the…
A: The tanning parlour is reffered as medical bed that is fitted with UV lights , and hence emits UV-A…
Q: Free radical formation can occur as a result of normal cellular metabolism? A. True B. False
A: Any molecular species capable of independent existence that has an unpaired electron in an atomic…
Q: Humans come in all sorts of skin colors. The difference is due to a O difference in levels of…
A: Humans come in all sorts of skin colors, every individual have a different skin color varying from…
Q: What mineral enhances the ability of insulin to transport glucose?
A: Chromium is a fundamental mineral that the body needs in follow sums. It is normally present in a…
Q: The three-nucleotide codon system can be arranged into ____________ combinations.a. 16b. 20c. 64d.…
A: Ribonucleic acid (RNA) is a nucleic acid found in both the nucleus and cytoplasm. Nucleotides are…
Q: QUESTION 46 A healthy weight loss plan includes all of the following, EXCEPT: O A. fast or skip…
A: Weight loss ideas : Stay hydrated Eat more fibre Eat in small amounts Limit sugary drinks and…
Q: Please explain prokaryotic translation.
A: Introduction Translation is the process by which ribosomes in the cytoplasm or endoplasmic…
Q: What system promotes efficient treatment of trauma patients? A. Emergency department information…
A: Introduction :- Trauma patients are people who suffer from trauma injuries. Trauma injuries are…
Q: ee radicals are very stable, which is why they accumulate in our body to cause agin
A:
Q: In protein synthesis, DNA transcription records the genetic message, while ribosomal ______________…
A: Molecular Biology is the branch of biology that deals with a study of the composition, arrangement,…
Q: QUESTION 39 Which is not a physical method of controlling microbial growth? O Sonication O…
A: Microorganisms are abundantly present in nature. They are either beneficial, neutral, or harmful to…
Q: If the food is contaminated by bacteria, as long as fully boiled for over 10 minutes, it is still…
A: Introduction Bacteria are single-celled organisms that are tiny. Bacteria can be found practically…
Q: QUESTION 5 The body's ability to heal itself after a cut would be.... O A Metabolism OB. A catabolic…
A: The question is asking about the process through which the body heals itself after a cut. This…
Q: Question 3. Eukaryote cells are living cells that are specialized to deal with changes in the…
A: The immune system checks the body for infections or problem-causing chemicals and fights any harmful…
Q: O B. is an organism that has genes in it from another organism.
A: The correct option is B . Is an organism that has genes from other organism .
Q: Match the terms with the numbers in the figure below b) Plantar view 2. 9. 8. 7. 4 6. 3. LO
A: Foot is the lowermost point of the human leg. The foot's shape help in keeping the body in a…
Q: Because _____ is colorless and odorless but can be highly toxic, it is important to have home…
A: Gases are of different types with different properties. Some have a specific color, some have a…
Q: What type of cells were concluded to be corrupted which constitutes a cell line when conducting…
A: The culture of the cells developed from the single cell that grows indefinitely with a uniform…
Q: Inoculation for bacterial growth in an agar petri dish requires A. Incolating one species of…
A: Spread plate method is a technique which uses a Petri plate or dish in which a growth medium or gar…
Q: Match the compounds on the left with the important roles they play listed on the right. (Answers are…
A: Prostaglandins is derived from arachidonic acid, contain a five carbon ring. Prostaglandins are…
Q: A dead human body demanding a high preservation amount of arterial injection fluid is a body showing…
A: The arterial injection fluid is given to a dead body into the arterial system. This fluid is also…
Q: The family of a patient who has just died asks to be alonewith the body and asks for supplies to…
A: When the family members lose their loved one this time is most crucial for them, they all are going…
Q: Medicare payment to HHRGS increases or decreases based on: A Resources used ASC rates Severity…
A: The main objective of a health care system is the well-being of people, and it can be done by…
Q: Question 11. What is the definition of “essential nutrients”? A. nutrients that are essential for…
A: Essential nutrients are one that cannot be synthesized by the body and therefore must be supplied…
Q: Question 29 A client was hospitalized with a foot wound that was healing poorly. The wound was the…
A: Vitamins and minerals play important role in growth and maintenance of health. There are some…
Q: QUESTION 20 All of the following are characteristics of pathogens that allow them to rapidly and…
A: The correct option is a Large genome size explanation:- The genome size doesn’t effect the pathogens…
Q: Vitamin D is naturally less in most food, therefore it is regularly obtained from fortified…
A: Vitamins are a collection of chemical substances that are absolutely necessary for the human body's…
Q: Which of these is the best reason for proper nutrition? A. To improve your intelligence B. To ensure…
A: Good nutrition refers to food that contains all of the required proteins, vitamins, and minerals for…
Q: Which is not an example of a euphemism? a. He passed away last year. b. Due to…
A: A euphemism is a polite, vague word or phrase that is used in place of word or phrase that might be…
Step by step
Solved in 2 steps
- In E. coli, Base excision repair (BER) handles non-bulky lesions (e.g., deamination, oxidation) and nucleotide excision repair (NER) handles bulky lesions (e.g., Py dimers). Is this statement true or false? O True FalsepMBBS plasmid was subjected to complete digestion by different combinations of three restrictive enzymes (EcoRI, BamHI, and XhoI), and the results analyzed on an agarose gel, Figure 1. Using the data, respond to the question below Draw the actual map of the pMBBS plasmid, following the style of the sample map shown in Figure Make sure you show the following information: (1) relative locations of the RE sites; (2) size in base pairs of the segments of DNA between sites. (The map need not be drawn to scale.)There can be more than one answer. Please help me
- b. Cleavage with chymotrypsin produces the following fragments: Band A: CN , NLQY, GIVEQCCHKRSEY Band B: F, Y, DPTKM, IACCVRGF, RTTGHLCGKDLVNALY Cleavage with Staphilococcus aureus V8 protease produces the following fragments: Band A: GIVE, YNLQNYCN, QCCHKRCSE Band B: PTKM, RTTGHLCGKD, LVNALYIACGVRGFFYD What is the amino acid sequence of the protein? Type your responseLast week in lab you plated various E. coli mutants. This week we will be analyzing those plates. To help interpret those results, predict the growth/color pattern you'd expect for a Lacz- mutant. Table 1: Options are blue or white for NA plates; Table 2: Options are: growth or none for minimal plates). Table 1 [Select] Table 2 [Select] Xgal glucose Xgal + lactose [Select] [Select] Minimal lactose ΝΑ [Select] Xgal + IPTG [Select] maltose > [SFor E. coli strains with the lac genotypes show below, use a plus sign (+) to indicate the synthesis of β-galactosidase and permease and a minus sign (–) to indicate no synthesis of the proteins.
- b. Neurospora has an arginine amino acid synthesis pathway shown below: ornithine citrulline arginine NH H2N H2N OH H2N HO HO NH2 NH2 ÑH2 Enzyme A Enzyme B Enzyme C ↑ ↑ Gene A Gene B Gene C Suppose I have a neurospora strain that has a mutation such that it will not grow unless I supplement the media (food) with arginine (but not with citrulline or ornithine). What gene is mutated? Explain your reasoning. C. Suppose I take the strain above that only grows with arginine supplements and cross it to a different mutant Neurospora strain that grows with arginine and citrulline supplements but not ornithine supplements. Assming genes A, B and C are unlinked and there is only one mutation per strain: i) What percentage of the progeny will grow on ornithine? ii) What percentage on citrulline? iii) What percentage on arginine? Show your work for i), ii) and iii). |Calculate these for 1 ug of plasmid DNA in a final total volume of 20 ul. All enzymes being used here are at a concentration of 10 U/µL. For double digests, give the appropriate volume for each enzyme and decide which buffer to use. (HINT: look up double digest conditions on the NEB website.) You should always calculate all volumes in advance to ensure the correct working concentrations and so that you can prepare the digests as efficiently as possible. It is a good idea to check off each component as it is added to the microfuge tube. Complete the table below, including the volume of DNA sample(s) you will need for 1 pg of the DNA (from two different methods in Experiment #3) based on the concentration(s) you determined from the OD260 value, which you will restriction digest with each of the restriction enzymes singly and in double digests in Part A. For example, the volume of DNA sample that contains 1 µg DNA for DNA sample from Part A, Experiment 3: OD260 = 0.024, dilution 500x…A pure culture of an unknown bacterium was streaked onto plates of a variety of media. You notice that the colony morphologyis strikingly different on plates of minimal media with glucose compared to that seen on trypticase soy agar plates. How can you explain these differences in colony morphology? Also, describe what happens when a nonsense mutation is introduced into the gene encoding transposase within a transposon and why is it more likely that insertions or deletions will be more detrimental to a cell than point mutations?
- TRUE OF FALSE: Is E coli colonies would be pink mucoid in Eosin Methylene BlueAgar (EMBA)? Is Coliform colonies would be pink mucoid in Eosin Methylene BlueAgar (EMBA)?In your laboratory, you have an F− strain of E. coli that is resistantto streptomycin and is unable to metabolize lactose, but it can metabolizeglucose. Therefore, this strain can grow on a medium thatcontains glucose and streptomycin, but it cannot grow on a mediumcontaining lactose. A researcher has sent you two E. colistrains in two separate tubes. One strain, let’s call it strain A, hasan F′ factor (an F prime factor) that carries the genes that are requiredfor lactose metabolism. On its chromosome, it also has thegenes that are required for glucose metabolism. However, it is sensitiveto streptomycin. This strain can grow on a medium containinglactose or glucose, but it cannot grow if streptomycin is addedto the medium. The second strain, let’s call it strain B, is an F−strain. On its chromosome, it has the genes that are required forlactose and glucose metabolism. Strain B is also sensitive to streptomycin.Unfortunately, when strains A and B were sent to you, thelabels had fallen…#1 HindII --- 5’ GTC ↓ GAC 3’ 5’ ACGACGTAGTCGACTTATTAT GTCGACCCGCCGCGTGTCGACCATCA 3’ 3’ TGCTGCATCAGCTGAATAATACAGCTGGGCGGCGCACAGCTGGTAGT 5’ Restriction enzyme: Recognition sequence: Number of pieces of DNA: Type of cut: