ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it nonfunctional, how would that affect the level of B-galactosidase protein activity with and without lactose present, why?

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter16: Adult And Pediatric Dosages Based On Body Surface Area
Section: Chapter Questions
Problem 7.2P
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Part ii. ) please
i.) Fill in the table for each of the E. coli: (0) = No Activity (+)= Basal Activity and (+++) = High Activity
E. coli chromosome
F Plasmid
B-gal activity?
Permease activity?
When
Lactose is
present
When
Lactose is
present
When
When
Glucose is
present
Glucose is
present
+++
+++
a.) I+ P+ O+ Z+ Y+
none
b.) I^[S] P+ O+ Z+ Y+
none
10
0.
c.) I+ P+ O^[c] Z+ Y+
none
+++
+++
+++
+++
d.) I+ P+ O- Z- Y+
0.
none
+++
+++
e.) I+ P+ O+ Z+ Y+
I^[S] P+ O+ Z+ Y+
f.) I[S] P+ O+ Z- Y+
+ P+ O^[c] Z+ Y-
9.) I^[TB] P+ O+ Z+ Y
I+ P+ O^[c] Z- Y+
h.) I+ P+ O^[c] Z+ Y-
I+ P+ O+ Z** Y+
i.) I^[TB] P+ O^[c] Z+ Y- 1+ P+ O+ Z- Y+
Z** is a polar mutation
ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it
nonfunctional, how would that affect the level of B-galactosidase protein activity with
and without lactose present, why?
Transcribed Image Text:i.) Fill in the table for each of the E. coli: (0) = No Activity (+)= Basal Activity and (+++) = High Activity E. coli chromosome F Plasmid B-gal activity? Permease activity? When Lactose is present When Lactose is present When When Glucose is present Glucose is present +++ +++ a.) I+ P+ O+ Z+ Y+ none b.) I^[S] P+ O+ Z+ Y+ none 10 0. c.) I+ P+ O^[c] Z+ Y+ none +++ +++ +++ +++ d.) I+ P+ O- Z- Y+ 0. none +++ +++ e.) I+ P+ O+ Z+ Y+ I^[S] P+ O+ Z+ Y+ f.) I[S] P+ O+ Z- Y+ + P+ O^[c] Z+ Y- 9.) I^[TB] P+ O+ Z+ Y I+ P+ O^[c] Z- Y+ h.) I+ P+ O^[c] Z+ Y- I+ P+ O+ Z** Y+ i.) I^[TB] P+ O^[c] Z+ Y- 1+ P+ O+ Z- Y+ Z** is a polar mutation ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it nonfunctional, how would that affect the level of B-galactosidase protein activity with and without lactose present, why?
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