Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 28, Problem 5P
Molecules of DNA Polymerase III per Cell vs. Growth Rate It is estimated that there are 40 molecules of DNA polymerase III per E. coli cell, is it likely that the growth rate of E. coli is limited by DNA polymerase III availability?
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The schematic diagram below shows organization of the DNA replication fork. Match parts of the diagram (labeled A-F) with the corresponding term from the answer list (designated
31
parental duplex
5'
3'
fork progression
v A
1Lagging strand
2. An Okazaki tragment
3.Site of action of DNA topoisomerase
4 Leading strand
5. Site of action of DNA helicCase
6.Site of action of DNA ligase
Asap please explain well.
Hh.136.
Chapter 28 Solutions
Biochemistry
Ch. 28 - Semiconservative or Conservative DNA Replication...Ch. 28 - The Enzymatic Activities of DNA Polymerase I (a)...Ch. 28 - Multiple Replication Forks in E. coli I Assuming...Ch. 28 - Multiple Replication Forks in E. coli II On the...Ch. 28 - Molecules of DNA Polymerase III per Cell vs....Ch. 28 - Number of Okazaki Fragments in E. coli and Human...Ch. 28 - The Roles of Helicases and Gyrases How do DNA...Ch. 28 - Human Genome Replication Rate Assume DNA...Ch. 28 - Heteroduplex DNA Formation in Recombination From...Ch. 28 - Homologous Recombination, Heteroduplex DNA, and...
Ch. 28 - Prob. 11PCh. 28 - Prob. 12PCh. 28 - Chemical Mutagenesis of DNA Bases Show the...Ch. 28 - Prob. 14PCh. 28 - Recombination in Immunoglobulin Genes If...Ch. 28 - Helicase Unwinding of the E. coli Chromosome...Ch. 28 - Prob. 17PCh. 28 - Functional Consequences of Y-Family DNA Polymerase...Ch. 28 - Figure 28.11 depicts the eukaryotic cell cycle....Ch. 28 - Figure 28.41 gives some examples of recombination...Ch. 28 - Prob. 21PCh. 28 - Prob. 22P
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- Multiple Replication Forks in E. coli II On the basis of Figure 28.2, draw a simple diagram illustrating replication of the circular E. coli chromosome (a) at an early stage, (b) when one-third completed, (c) when two-thirds completed, and (d) when almost finished, assuming the initiation of replication at oriC has occurred only once. Then, draw a diagram showing the E. coli chromosome in problem 3 where the E. coli cell is dividing every 20 minutes.arrow_forward9a pleasearrow_forwardPlease help with 2a) 2a) There are two different DNA polymerase enzymes, DNA Polymerase I and DNA PolymeraseIII, that are active during prokaryotic DNA replication. Suppose you generated a mutant E. colistrain in which DNA Polymerase III was inactivated (all its enzymatic activities were non-functional) - assuming that all the other enzymes involved in replication remained fullyfunctional, how would DNA replication in these mutant cells without DNA Pol III differ fromDNA replication in normal E. coli? Briefly explain why you would expect to see thatchange/those changes in DNA replication in the mutant cells.arrow_forward
- Which of the following phases is characterized by preparation for DNA synthesis? G0 G1 G2 Sarrow_forwardMultiple Replication Forks in E. coli I Assuming DNA replication proceeds at a rate of 750 base pairs per second, calculate how long it will take to replicate the entire E. coli genome. Under optimal conditions, E. coli cells divide every 20 minutes. What is the minimal number of replication forks per E. coli chromosome in order to sustain such a rate of cell division?arrow_forwardPart ii. ) pleasearrow_forward
- There can be more than one answer. Please help mearrow_forwardRegulation of Genes and Their products 1. Given the following genotypes, explain how the mutation (identified by a (-) superscript) wil affect E. coll grown in lactose medium. Will the lac operon be on or off? Will there be a complete set of gene products from the lac operon? What will be the implication of the missing gene product, if ever? Will the cell be able to survive in the lactose medium or not? a. I+p+o+z- y+ b. i- p+o+z+y+ c. i+p+o- z+y+ d. i+p- o+z+y+ 2. In terms of the trp operon, differentiate between two normal bacterial cultures, one grown in a medium supplied with tryptophan and the other medium without tryptophan. 3. Experiments show that mutations at gene E lead to non-repressible transcription of trp genes. Why?arrow_forwardDisdis -how do RB and p53 negatively regulate the cell cycle in G1? describe the events that occur in the initiation and elongation of DNA replication in S phase.arrow_forward
- Pretty sure I answered this wrong. Please helparrow_forwardMultiple choice 1.) An Action potential A. is essential for nerve impulse propagation B. involves the influx of negative ions to depolarize the membrane C. involves the outflux of negative lons to depolarize the membrane D. Involves the outflux of positive ions to depolarize the membrane 2.) Which function can be carried out by DNA replication proteins? A. Topoisomerases wind the DNA into a double-helix. B. DNA ligase can initiate new DNA chains C. SSB converts double-stranded DNA into single-stranded DNA. D. Helicases break hydrogen bonds in the DNA. 3.) Which mechanism contributes to accuracy during DNA replication? A. The mismatch repair system recognizes an incorrect base-pair and corrects the mistake in the non-methylated strand. B. Using primers increases accuracy because the first nucleotides in a new nucleic acid chain are more likely to be correct. C. All DNA polymerases have a 5'-3' exonuclease activity which can remove incorrect nucleotides during…arrow_forwardMatching Type Choose the directionality of the given process. (4 points) What is the directionality of the given process? * 4 points 3'-5' 5'-3' Exonuclease activity Complementary strand of the continuous strand Addition of nucleotides going to the replication fork Addition of nucleotides away from the replication forkarrow_forward
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