Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 28, Problem 22P
Interpretation Introduction
Interpretation:
To explain the interaction of RFC with PCNA to recognize primer-template junction during
Concept introduction:
DNA or Deoxyribonucleic acid is a molecule made of two chains which coil around one another. These form a double helix which carries instructions genetical in nature like related to reproduction, growth, development, functioning of the living organisms.
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From standpoint of replication and transcription, explain how RNA polymerase is allowed to incorporate the first nucleotide whereas DNA polymerase needs a primer. Explain how this difference impacts the process of replication and transcription.
Matching Type
Choose the directionality of the given process. (4 points)
What is the directionality of the given process? *
4 points
3'-5'
5'-3'
Exonuclease activity
Complementary strand of the
continuous strand
Addition of nucleotides going
to the replication fork
Addition of nucleotides away
from the replication fork
All known DNA polymerases catalyze synthesis only in the 5' → 3' direction. Nevertheless, during semiconservative DNA replication in the cell, they are able to catalyze the synthesis of both daughter chains, which would appear to require synthesis in the 3' → 5' direction on one strand. Explain the process that occurs in the cell that allows for synthesis of both daughter chains by DNA polymerase
Chapter 28 Solutions
Biochemistry
Ch. 28 - Semiconservative or Conservative DNA Replication...Ch. 28 - The Enzymatic Activities of DNA Polymerase I (a)...Ch. 28 - Multiple Replication Forks in E. coli I Assuming...Ch. 28 - Multiple Replication Forks in E. coli II On the...Ch. 28 - Molecules of DNA Polymerase III per Cell vs....Ch. 28 - Number of Okazaki Fragments in E. coli and Human...Ch. 28 - The Roles of Helicases and Gyrases How do DNA...Ch. 28 - Human Genome Replication Rate Assume DNA...Ch. 28 - Heteroduplex DNA Formation in Recombination From...Ch. 28 - Homologous Recombination, Heteroduplex DNA, and...
Ch. 28 - Prob. 11PCh. 28 - Prob. 12PCh. 28 - Chemical Mutagenesis of DNA Bases Show the...Ch. 28 - Prob. 14PCh. 28 - Recombination in Immunoglobulin Genes If...Ch. 28 - Helicase Unwinding of the E. coli Chromosome...Ch. 28 - Prob. 17PCh. 28 - Functional Consequences of Y-Family DNA Polymerase...Ch. 28 - Figure 28.11 depicts the eukaryotic cell cycle....Ch. 28 - Figure 28.41 gives some examples of recombination...Ch. 28 - Prob. 21PCh. 28 - Prob. 22P
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- Mention two functions of DNA polymerase I in E. coli replication machinery?arrow_forwardDescribe an experimental approach to determining the processivity of a DNA polymerase (that is, the number of nucleotides incorpo- rated per chain per polymerase binding event).arrow_forwardUsing the numbered sites on the DNA double helix strands below, where would the DNA Primer ACTTGCGA bind to for DNA Amplification?arrow_forward
- a) Explain how the molecular mechanism of DNA polymerase enhances DNA replication. b) Discuss the characteristic of DNA polymerase 1, Nick translation Proofreadingarrow_forwardImagine a bacterial cell with a mutation that renders helicase completely nonfunctional (note that this would be a lethal mutation). What, precisely, would go wrong with replication in this cell? Please describe what goes wrong, as well as all downstream effects.arrow_forward"DNA polymerase I serves a secondary function in vivo,now believed to be critical to the maintenance of fidelityof DNA synthesis" Explain this statement ?arrow_forward
- PRDM9 recruits SPO11, a topoisomerase type II like protein for inducing DNA double strand breaks. Illustrate how SPO11 releases SPO11-linked oligonucleotides. rarrow_forwardExplain the molecular mechanism of DNA polymerization by DNA polymerase and explain why DNA polymerase 3 and not dna polymerase 1 is responsible for replicating the bacterial genome.arrow_forwardDetermine the complementary strand of DNA that forms on this template DNA fragment during replication: 5′GGTTTCTTCAAGAGA3′arrow_forward
- (a) How fast does template DNA spin (expressed in revolutions per second) at an E. coli replication fork? (b) What is the velocity of movement (in micrometers per second) of DNA polymerase III holoenzyme relative tothe template?arrow_forwardA mixture of four a-[*p]-labeled ribonucleoside triphosphates was added to permeabilized bacterial cells undergoing DNA replication in the presence of an RNA polymerase inhibitor, and incorporation into high-molecular- weight material was followed over time, as shown in the accompanying graph. After 10 minutes of incubation, a 1000-fold excess of unlabeled ribo- nucleoside triphosphates was added, with the results shown in the graph.arrow_forwardExplain how DNA polymerase and topoisomerase 2 contribute to replication in E.coli and what is the role of the role of the metal ions in the polymerase activity. B)How does the use of an RNA primer rather than a DNA primer affect the fidelity of DNA replication in E.coli?arrow_forward
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