Biochemistry
Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 28, Problem 17P
Interpretation Introduction

Interpretation:

To devise an experiment to demonstrate the displacement of DNA polymerase III by DNA polymerase IV.

Concept introduction:

DNA or Deoxyribonucleic acid is a molecule made of two chains which coil around one another. These form a double helix which carry instructions genetical in nature like related to reproduction, growth, development, functioning of the living organisms.

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Please help with 2a) 2a) There are two different DNA polymerase enzymes, DNA Polymerase I and DNA PolymeraseIII, that are active during prokaryotic DNA replication. Suppose you generated a mutant E. colistrain in which DNA Polymerase III was inactivated (all its enzymatic activities were non-functional) - assuming that all the other enzymes involved in replication remained fullyfunctional, how would DNA replication in these mutant cells without DNA Pol III differ fromDNA replication in normal E. coli? Briefly explain why you would expect to see thatchange/those changes in DNA replication in the mutant cells.
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