You perform two single digests on the plasmid below, named pPER, with the restriction enzymes EcoRI and BamHI. One fragment, 6,000 base pairs in size, will result if the plasmid is digested with BamHI, which is illustrated in lane 2. The marker is in lane 4 and the sizes of markers are to the right of the gel in base pairs (bp). EcoRI, 5000- 0, 6000 PPER wwwwwwww 6000bp BamHI, 3000 EcoRI, 1000 Use the figures to answer the following questions. Question 3 1 EcoRI BamHI 2 4 ABC - -10,000 bp - 8,000 -6,000 -5,000 - 4,000 -3,000 D - 2,000 1,500 E 1,000 500 For the EcoRI single digest, determine the number and size of the resulting fragments by choosing the correct locations (letters) on the gel above.
You perform two single digests on the plasmid below, named pPER, with the restriction enzymes EcoRI and BamHI. One fragment, 6,000 base pairs in size, will result if the plasmid is digested with BamHI, which is illustrated in lane 2. The marker is in lane 4 and the sizes of markers are to the right of the gel in base pairs (bp). EcoRI, 5000- 0, 6000 PPER wwwwwwww 6000bp BamHI, 3000 EcoRI, 1000 Use the figures to answer the following questions. Question 3 1 EcoRI BamHI 2 4 ABC - -10,000 bp - 8,000 -6,000 -5,000 - 4,000 -3,000 D - 2,000 1,500 E 1,000 500 For the EcoRI single digest, determine the number and size of the resulting fragments by choosing the correct locations (letters) on the gel above.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:You perform two single digests on the plasmid below, named pPER, with the restriction
enzymes EcoRI and BamHI. One fragment, 6,000 base pairs in size, will result if the plasmid is
digested with BamHI, which is illustrated in lane 2. The marker is in lane 4 and the sizes of
markers are to the right of the gel in base pairs (bp).
EcoRI, 5000-
0, 6000
PPER
wwwwwwww
6000bp
BamHI, 3000
EcoRI, 1000
Use the figures to answer the following questions.
Question 3
1
EcoRI BamHI
2
4
ABC
-
-10,000 bp
- 8,000
-6,000
-5,000
- 4,000
-3,000
D
- 2,000
1,500
E
1,000
500
For the EcoRI single digest, determine the number and size of the resulting fragments by
choosing the correct locations (letters) on the gel above.
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