A student wishes to transform some E. coli with a plasmid to “knock-out” (ie. “silence” or prevent) the activity of Gene X, a gene important in the metabolism of Nutrient X. If Gene X is expressed, it can metabolize Nutrient X, contained within the agar plates, to produce neon orange colonies. To perform this transformation, the student knows that a plasmid must be used. You can assume that the materials this student has to perform their transformation are the same as those you had to complete lab #3. With the above in mind, please answer the followinga) What is a plasmid and why does this student need to use one to perform their transformati b) How should the student build their plasmid? Identify and briefly describe three key components they should include in their plasmid. c) Explain how the student can make their bacterial cells competent to receive their plasmid DNA. (3 pts.)

The transformation is the natural process in which the donor bacteria releases the free DNA in the…

Answered: A student wishes to transform some E.…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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A student wishes to transform some E. coli with a plasmid to “knock-out” (ie. “silence” or prevent) the activity of Gene X, a gene important in the metabolism of Nutrient X. If Gene X is expressed, it can metabolize Nutrient X, contained within the agar plates, to produce neon orange colonies. To perform this transformation, the student knows that a plasmid must be used. You can assume that the materials this student has to perform their transformation are the same as those you had to complete lab #3. With the above in mind, please answer the following
a) What is a plasmid and why does this student need to use one to perform their transformati

b) How should the student build their plasmid? Identify and briefly describe three key components they should include in their plasmid.

c) Explain how the student can make their bacterial cells competent to receive their plasmid DNA. (3 pts.)

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