Plasmid A carries an ampicillin resistant gene (bla) and a gene for production of a GFP protein; this protein allows bacteria to fluoresce green when irradiated with UV light. If we transform a bacteria that does not have either of these genes or traits with plasmid A, the bacteria transformed with plasmid A should now (select both correct options). grow on plates containing kanamycin grow on plates containing ampicillin not fluoresce when irradiated with UV light fluoresce blue when irradiated with UV light fluoresce green when irradiated with UV light

Plasmid A carries an ampicillin resistant gene (bla) and a gene for production of a GFP protein; this protein allows bacteria to fluoresce green when irradiated with UV light. If we transform a bacteria that does not have either of these genes or traits with plasmid A, the bacteria transformed with plasmid A should now (select both correct options). grow on plates containing kanamycin grow on plates containing ampicillin not fluoresce when irradiated with UV light fluoresce blue when irradiated with UV light fluoresce green when irradiated with UV light

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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In an attempt to clone a gene into the TOL plasmid, it was observed that the restriction product of the gene of interest were blunt ended, thereby reducing the ligation efficiency of this gene and the TOL plasmid. Elucidate two methods that could be employed to increase the ligation efficiency of your restriction products.
PCHEM4321. An agarose gel electrophoresis pattern of the plasmid PSPM4321 digestion (restriction) is shown below. Draw a restriction map of a plasmid with the appropriate restriction sites based on the data given below. Hindlll Hindll BamHI +BamHI Figure 1: 1% agarose gel electrophoresis of pCHEM4321 40 24 16 12 12 8 4 4 + |
You utilised two plasmids in this practical, pOTC and pOTC-Δ. Plasmids are often represented using plasmid maps like the one below. This map shows the positions of recognition sites for a number of restriction enzymes Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if this plasmid was digested with restriction enzymes EcoRI and PvuII.
Your plasmid miniprep has a concentration of 25 ng/µL and you add 30 µL to a digest tube. Your final reaction volume will be 50 µL. What is your final plasmid concentration in ng/µL?
You are on a research project to study rice. Your want to use reverse transcription PCR to amplify the OsMEI28 cDNA from the plant and create a plasmid that contains the cDNA sequence in a vector. Briefly describe the steps that you would need to take.
For A. Answer is Culture in ampicillin media to isolate bacteria that took up the plasmid, killing off the bacteria that did not take up the plasmid. Can you explain why? For B. Answer is Lane C. Can you explain why?
A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic regions. The insect’s genomic DNA has been treated with the restriction enzyme EcoRI. One of the fragments (with a length of 4 kb) produced in this manner is isolated and ligated into a pUC19 plasmid vector that has also been treated with EcoRI. This recombinant construct is called plasmid A. Samples of plasmid A, pUC 19, and the total genomic DNA from the insect are digested completely with the restriction enzyme EcoRI. The three digests are loaded into separate wells: plasmid A (lane 1), pUC19 (lane2) and total insect genomic DNA (lane 3) of an agarose gel and subjected to electrophoresis. 2b. Draw what will be seen in each lane if the gel is transferred to a membrane and subjected to Southern blotting and the 4000 base pair genomic DNA fragment (labeled with a fluorescent tag) from the insect is used to probe the blot.
In an restriction enzyme experiment where Eco RI and Hind III are used . What would happen if a third restriction enzyme, BamHI were used in this lab if there was only one site on the plasmid that is recognized by BamHI? Explain what difference you would see in the gel
A.) Transformation is best described as: Group of answer choices The integration of foreign DNA into a genome The uptake of naked DNA from the environment The transfer of DNA via a bacteriophage Transfer of a plasmid from one organism to another B.) What is the function of the araC gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on to daughter cells C.) What is the function of the bla gene in the pGLO plasmid? Group of answer choices It encodes the protein that glows under ultraviolet light It allows us to select for the cells that contain the plasmid It prevents the transcription of the green fluorescent protein unless arabinose is added It ensures that the plasmid will be copied and passed on…
Competent E. coli cells were transformed with the pGLO plasmid. These transformed cells were then allowed to grow on two different plates: 1) a plate containing LB/AMP and 2) another plate containing LB/AMP/ARA. In which plate would you observe both phenotypic and genotypic changes? Briefly justify your answer. Edit View Insert Format Tools Table 12pt v Paragraph v BIUA e T?v 田 D2
Why do we need a plasmid to transform a bacteria? What are the essential components of a plasmid and explain their functions. What is the difference between a Plasmid and a Vector?
You first need to create a plasmid. What are the minimal components necessary to develop this plasmid? In addition to these components, please draw the plasmid. In this illustration, I am looking for the components, the direction of transcription (i.e., the direction the genes will be transcribed), and what should be transcribed last? Also, where would you specifically insert the P. falciparum gene in this plasmid, and why are you checking reading frames in your gene? Finally, please name your plasmid using the correct nomenclature too. You are excited you have this new plasmid; you want to transfect it into P. marinus and provide it as an oral vaccine to laboratory mice. However, even though your supervisor enjoys your enthusiastic attitude, they do not allow you to do this quite yet because all you have is this plasmid. Why doesn’t your supervisor allow you to use the laboratory mouse for this research regarding animal welfare guidelines? Your answer should include the 3 R’s of…