A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic regions
A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic regions. The insect’s genomic DNA has been treated with the restriction enzyme EcoRI. One of the fragments (with a length of 4 kb) produced in this manner is isolated and ligated into a pUC19 plasmid vector that has also been treated with EcoRI. This recombinant construct is called plasmid A. Samples of plasmid A, pUC 19, and the total genomic DNA from the insect are digested completely with the restriction enzyme EcoRI. The three digests are loaded into separate wells: plasmid A (lane 1), pUC19 (lane2) and total insect genomic DNA (lane 3) of an agarose gel and subjected to electrophoresis.
2a. Draw a gel (you can do it by hand) showing what will be seen in the lanes 1, 2, and 3 of the gel after it is stained with ethidium bromide for visualizing DNA. (indicate molecular sizes where appropriate)
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