an insert check (labeled A-D below). After growing cultures, you isolate the plasmids and treat them with EcoRI. You then run them on a are white? BCD B and C and C and C I are white and C Ladder 2kb 1kb 200bp

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You are researching a gene that is 500bp long called rad. You know the gene is between two EcoRI restriction sites. You treat genomic DNA with EcoR1 and
you treat your pUC-19 plasmid with EcoRI and ligate the genomic fragments into your vector. You use blue/while selection and pick four colonies and you
perform an insert check (labeled A-D below). After growing cultures, you isolate the plasmids and treat them with EcoRI. You then run them on a gel. Which
colonies are white?
ABC D
0
O a. A, B and C
b. B and C
O c. B and C
O d. All are white
O e. A and C
Ladder
2kb
1kb
200bp
Transcribed Image Text:You are researching a gene that is 500bp long called rad. You know the gene is between two EcoRI restriction sites. You treat genomic DNA with EcoR1 and you treat your pUC-19 plasmid with EcoRI and ligate the genomic fragments into your vector. You use blue/while selection and pick four colonies and you perform an insert check (labeled A-D below). After growing cultures, you isolate the plasmids and treat them with EcoRI. You then run them on a gel. Which colonies are white? ABC D 0 O a. A, B and C b. B and C O c. B and C O d. All are white O e. A and C Ladder 2kb 1kb 200bp
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