an insert check (labeled A-D below). After growing cultures, you isolate the plasmids and treat them with EcoRI. You then run them on a are white? BCD B and C and C and C I are white and C Ladder 2kb 1kb 200bp

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
icon
Concept explainers
Question
You are researching a gene that is 500bp long called rad. You know the gene is between two EcoRI restriction sites. You treat genomic DNA with EcoR1 and
you treat your pUC-19 plasmid with EcoRI and ligate the genomic fragments into your vector. You use blue/while selection and pick four colonies and you
perform an insert check (labeled A-D below). After growing cultures, you isolate the plasmids and treat them with EcoRI. You then run them on a gel. Which
colonies are white?
ABC D
0
O a. A, B and C
b. B and C
O c. B and C
O d. All are white
O e. A and C
Ladder
2kb
1kb
200bp
Transcribed Image Text:You are researching a gene that is 500bp long called rad. You know the gene is between two EcoRI restriction sites. You treat genomic DNA with EcoR1 and you treat your pUC-19 plasmid with EcoRI and ligate the genomic fragments into your vector. You use blue/while selection and pick four colonies and you perform an insert check (labeled A-D below). After growing cultures, you isolate the plasmids and treat them with EcoRI. You then run them on a gel. Which colonies are white? ABC D 0 O a. A, B and C b. B and C O c. B and C O d. All are white O e. A and C Ladder 2kb 1kb 200bp
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps

Blurred answer
Knowledge Booster
Bacterial genomics
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education