EcoRI aloneHindIll aloneBam HI aloneEcoRI+HindillEcoRI + BamHIHindIll+ BamHI15,000 bp15,000 bp15,000 bp7,000 and 8,000 bp4,000 and 11,000 bp4,000 and 11,000 bpAmpⓇPUKY plasmid15,000 bp2,000EcoRIinsert7,000Questions 27-29:plasmid pUKY shown above. The insert contains one EcoRI site but no recognition sites for Hindill orYou have cloned a DNA fragment intoBamHI. The position of the EcoRI site is indicated on the figure below. The extent of the inserted DNA fragment is indicated by the striped bar. Youperform several restriction digests of the recombinant DNA, using each enzyme alone or in combination with another enzyme. The sizes of theresulting restriction fragments are shown in the table.At what position number on the plasmid is the BamHI site?

Answered: EcoRI alone HindIII alone Bam HI alone…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

EcoRI alone
HindIll alone
Bam HI alone
EcoRI+Hindill
EcoRI + BamHI
HindIll+ BamHI
15,000 bp
15,000 bp
15,000 bp
7,000 and 8,000 bp
4,000 and 11,000 bp
4,000 and 11,000 bp
AmpⓇ
PUKY plasmid
15,000 bp
2,000
EcoRI
insert
7,000
Questions 27-29:
plasmid pUKY shown above. The insert contains one EcoRI site but no recognition sites for Hindill or
You have cloned a DNA fragment into
BamHI. The position of the EcoRI site is indicated on the figure below. The extent of the inserted DNA fragment is indicated by the striped bar. You
perform several restriction digests of the recombinant DNA, using each enzyme alone or in combination with another enzyme. The sizes of the
resulting restriction fragments are shown in the table.
At what position number on the plasmid is the BamHI site?

Expert Solution

Step 1: Restriction digestion

Restriction digestion, also known as restriction endonuclease digestion or simply restriction cleavage, is a laboratory technique used in molecular biology to cut DNA molecules at specific locations. It relies on the use of enzymes called restriction endonucleases, which are naturally occurring proteins produced by bacteria as part of their defense mechanisms against foreign DNA, such as viral DNA.

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