Which of these plates are the control plates? What purpose does a control plate serve? On which plates did you expect to find transformants? Explain what a single colony represents on the petri dish labeled + LB/AMP.

Q: You are studying a microorganism that contains a chloramphenicol acetyltransferase (CAT) enzyme, and…

A: As parent have CAT enzyme means it grows on chloramphenicol containing plate. And we want clone with…

Q: If  a flask is inoculate with 10 E. coli cells. After 6 hours you now have 200,000 cells. determine…

A: Bacteria shows exponential growth curve. The growth of the bacteria can be calculated by using the…

Q: The PCB concentration in Lake Michigan is declining. The concentration can be modeled using a first…

A: The first order reaction is a reaction that proceeds at the rate that depends linearly on only one…

Q: Bacillus subtilis is known to harbour proA gene which is expressed as protease. Protease is an…

A: Genetic engineering and bioprocess are very good tools in the pharmaceutical, therapeutics, enzyme…

Q: You perform a plaque assay with your bacteria and serial dilutions of your bacteriophage,, and then…

A: Serial dilution is a process of making a series of dilution in order to determine the concentration…

Q: FRET is a technique that measures the transference of energy between a donor and an acceptor…

A: A widely used fluorescence technique to study bimolecular interactions within cells is FRET, which…

Q: Differential centrifugation can be improved through the use of a density gradient. What is the…

A: In scientific experiments, various techniques are used to isolate, measure or quantify samples based…

Q: Each Escherichia coli cell weighs 5ng. How many cells are present in a colony that weighs 1mg? Write…

A: Escherichia coli is a Gram-negative bacteria. It is present as normal intestinal flora but can also…

Q: Bacillus thuringiensis makes toxins that kill insects. These toxins must be applied several times…

A: Bacillus thuringiensis is responsible for the production of toxins that are capable of killing…

Q: During the Lederberg and Tatum experiment, theresearchers compared the growth of mutant strains…

A: Conjugation is the process by which bacteria transfers its genetic material to the other bacteria by…

Q: You then take a cotton swab and subculture a sample from this zone of inhibition (where no growth…

A: The Kirby-Bauer test, known as the disk-diffusion method, is the most widely used antibiotic…

Q: What is the role of Agrobacterium tumefaciens in plant transformation?

A: The bacterium Agrobacterium tumefaciens is responsible for causing the crown gall disease in plants.…

Q: In order to transform a bacteria, the cell wall should be perforated either through physical or…

A: The cell wall is the very outer membrane of the cell. It has a rigid, semi-permeable protective…

Q: Why are there just colonies and not a lawn of growth on plates (+) LB/AMP/ARA and (+)LB/AMP?

A: The LB+/AMP and LB + AMP/ARA represents the experimental plates having the antibiotic ampicillin in…

Q: Researchers studying the bacterium Escherichia coli split a population of the bacteria into two…

A: Bacteria are very adaptable creatures with the unique capacity to take in and reproduce (or copy)…

Q: Question 11: If mutant haploid strain is mixed with a wild-type haploid strain, how many chromosomes…

A: Haploid and diploids An organism is said to be haploid when it consists of a single copy of a…

Q: If a bacterial species is not susceptible to an antibacterial drug at the concentration present in a…

A: Antibiotic susceptibility test is the test used to determine the effect of antibiotic to an…

Q: Imagine you made a single kirby bauer plate to determine if a given antibiotic were effective for…

A: Introduction Antibiotics, also called antibacterials, are drugs that kill or slow the growth of…

Q: You are a technician working in a microbiology lab. You grow two bacteria cultures, Bug A and Bug B,…

A: The cell culture medium is a liquid nutrient solution used to grow and maintain cells in vitro. It…

Q: What is the protocol of the yeast active experiment

A: Active yeast refers to live yeast cells that are capable of fermentation, which is the process of…

Q: What are the concepts behind osmosis and enzymatic browning in potatoes?

A: Osmosis is the movement of water or other solvents across a semi permeable membrane from a region of…

Q: Why is it important to incubate plates upside down (inverted)?

A: INTRODUCTION Why Petri plates are incubated upsidedown To avoid contamination.

Q: What is the role of the following in the alkaline plasmid screen?    G buffer (Cell Suspension…

A: G buffer (Cell suspension solution): It is a solution that consists of a mixture of Tris (pH 7.5)…

Q: Doug and Virginia wanted to find out if temperature affects the growth of mould on bread. At lunch,…

A: In the earlier times, the growth of moulds on bread is used as inadvertent natural clocks as almost…

Q: Each Escherichia coli cell weighs 5ng. How many cells are present in a colony that weighs 1mg?

A: E. coli is a rod-shaped, Gram-negative, facultative anaerobic coliform bacteria of the genus…

Q: You are working with a yeast that can undergo fermentation or respiration. You take equal aliquots…

A: Introduction - Fermentation in yeasts produces ethanol and carbon dioxide, both of which can be used…

Q: They want to determine if the samples contain a toxin or a virus. Material from each sample is used…

A: Sample 1 contains a virus and sample 2 contains a toxin. This choice is based on the fact that the…

Q: Mention and explain at least one reason why you might obtain growth of bacteria on EMB that was…

A: EMB is selective dye used for isolation of gram negative bacterial ( E.coli and coliforms)   EMB…

Q: Name the two plant which are produced by micropropagation?

A: Micropropagation is a rapid vegetative proportion technique to increase the number of plants with…

Q: Describe ,A Hfr bacterium mates with an F- plasmid containing bacterium. What

A: PLASMIDS- It was discovered that maleness in bacteria is determined by a transmissible genetic…

Q: A media contains 2 E. coli cells which have a generation time and a replication time of 60 and 40…

A: Given: 2 E.coli cells generation time = 60 mins. 2 E.coli replication time = 40 mins. Number of…

Q: 0.2 mL of YGM was added to 1.8 mL of 0.2% YGM. What is the % concentration of yeast in this sample?

A: % concentration is calculated by C1V1=C2V2.

Q: Fifteen bacterial colonies growing on a complete medium (that means that they have all of nutrients…

A: Bacteria are capable of synthesizing all the 20 amino acids. In a complete medium, all the nutrients…

Q: THIS IS A TWO PART QUESTION FROM THE SAME DATA. PLEASE ANSWER BOTH The two graphs below are 2 sets…

A: Most bacteria's cell walls can be divided into two categories, gram-positive and gram-negative. In…

Q: You have several different media onto which you inoculated eight strains of yeast (A-H). The media…

A: Minimal medium (unsupplemented) A minimal medium is a growth medium used for growing microbes and…

Q: Assume that you counted 67 plaques on a bacterial plate where 0.1ml of a 10-5 dilution of phage was…

A: A dilution: It can be defined as a method of addition of more solvent to a solution of a definite…

Q: Researchers studying the bacterium Escherichia coli split a population of the bacteria into two…

A: The claim best supported by the data in table 1 is: D. Only the bacteria that were successfully…

Q: Please indicate whether you expect growth or no growth. (Each cell is worth 0.25) Tetracycline No…

A: Antibiotic resistance is a normal part of life. Antibiotic resistance, on the other hand, is driven…

Q: lease describe a strategy to correctly balance a centrifure rotor if you have 4 samples. 2 samples…

A: Centrifuge: It is an important tool in the biochemistry lab that through a rapid spinning process…

Q: How can i explain my answers in depth in microbology for example this question: Assume that there…

A: Horizontal gene transfer is the transfer of genome from one individual to another which may or may…

Q: What is the difference between a bactericidal antibiotic and an antibiotic that is bacteriostatic?…

A: Microbes are organisms that cannot be seen as such through naked eyes but require microscope for…

Q: ____________ help to transfer DNA between bacterial cells during the conjugation.

A: Conjugation is a type of horizontal gene transfer. It refers to the transfer of genetic material…

Q: A total of 2.5 x 105 cells were harvested from the patient. You need the cells to fill a scaffold…

A: For calculation of volume we need to know the basic formula of l×b×h

Q: How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume?…

A: Dilution factor refers to the ratio of the volume of the initial (concentrated) solution to the…

Q: rains of E. coli are isolated. They may be mutated on the ara, mal, leu and pro genes, only these 4…

A: Microorganisms can grow in culture medium containing different nutrients one of those nutrients…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.