Draw a dilution scheme for a dilution of phage from 1.5 x 1010 PFU/mL to 1.00 x 102 PFU/mL. Achieve this serial dilution considering the following: The volumes of each dilution should be 1.0mL. It is not advisable to perform any dilutions > 1/100. The first dilution in any titre determination of stock phage should use 10µl or less of stock.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Draw a dilution scheme for a dilution of phage from 1.5 x 1010 PFU/mL to 1.00 x 102 PFU/mL. Achieve this serial dilution considering the following:
- The volumes of each dilution should be 1.0mL.
- It is not advisable to perform any dilutions > 1/100.
- The first dilution in any titre determination of stock phage should use 10µl or less of stock.
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