You have a cell count of 6.7 x 10° cells / ml in a total volume of 4.5ml. You have resuspended your cells in 5ml sterile PBS. You need to seed a six-well plate with the following numbers: a. 2 wells at 1 x 10" cells / well (2ml / well) b. 2 wells at 4 x 10 cells /well (2ml / well) c. 2 wells at 2 x 10° cells / well (2ml / well) Determine the volume of the original culture you will need to seed each concentration.

You have a cell count of 6.7 x 10° cells / ml in a total volume of 4.5ml. You have resuspended your cells in 5ml sterile PBS. You need to seed a six-well plate with the following numbers: a. 2 wells at 1 x 10" cells / well (2ml / well) b. 2 wells at 4 x 10 cells /well (2ml / well) c. 2 wells at 2 x 10° cells / well (2ml / well) Determine the volume of the original culture you will need to seed each concentration.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Which of the following is false about counting viable cells? A. CFU(Colony forming unit) are counted instead of individuals cells B. Only 30-300 CFUs are statistically valid C. Colonies growing on the plate represent a fraction of the live cells in the original sample D. You can determine the number of live cells in a broth culture E. It is necessary to multiply the by CFUs to determine the number of viable cells in the original sample
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After trypsinization of cells in the flask and cell counting, you determined a concentration of 10 million cells/mL. What volume of this cell suspension will you use to prepare a 20 mL cell suspension (200,000 cells/mL) for a 24-well plate? O a. 0.4 ul b.0.4 mL Oc. 40 mL O d.40 µL e. none of the above
You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?
Thirty corn seedlings of equal size were randomly assigned to one of three treatment groups. At the beginning of the experiment, the plants in group I were dried and the mass was determined. The plants in group Il were maintained in light for a week. The plants in group III were maintained in the dark for a week. All conditions, other than light, were the same for groups IlI and III. At the end of the week, the plants in groups Il and III were dried and the mass was determined. The experimental results are provided in the table. Initial Dry Mass of 10 Plants (g) Dry Mass of 10 Plants After One Week (g) Change in Dry Mass of 10 Plants Over One Week (g) Treatment Group Treatment Nohe 14.8 II Light 32.8 +18 III Dark 11.7 -3.1 (a) To explain the increase in mass of the light-grown plants, identify ONE inorganic source of new plant mass and connect it to the cellular process underlying the increase in mass. (b) To explain the decrease in mass of the dark-grown plants, identify the overall…
You need linear-linear paper and log-linear paper (also called semi-log paper), for linear-linear,10 squares per inch. For log-linear = semi-log you need at least 3 full cycles. Plot the values below on each type of paper, then select two points marking an approximate doubling of cell density and interpolate back to the ‘x’ axis to determine the time. Indicate the bracketing for your estimate, and state ‘g= ____ hr’ Time (minutes) OD420 0 0.091 20 0.092 40 0.111 60 0.152 80 0.208 100 0.303 120 0.430 140 0.577 160 0.732 180 0.902 200 1.001 220…
Table 1a Why Are Cells Small Length (l) (cm) Width (w) (cm) Height (h) (cm) Size of Cross Section (h x w) (cm) Distance Traveled by IKI from Potato Edge (cm) Area of White Region (l x w) (cm) 2.50 2.50 2.50 6.25 cm 0.20 cm 2.64 cm 2.00 2.00 1.00 2.00cm 0.30 cm 1.87 cm 1.50 1.50 1.50 2.25 cm 0.50 cm 1.50 cm 1.00 1.00 1.00 1.00 cm 0.40 0.60 cm 2.00 0.50 0.50 0.25 cm 0.20 cm 0.15 cm 0.50 0.50 0.50 0.25 cm 0.30 cm 0.10 cm help to fill the table