3) If Hybridoma cells are cultured supported in an agarose matrix in which nutrients and oxygen are supplied throughthin equi-distanced parallel tubes that pass longitudinally along the agarose tissue bundle, name two appropriateboundary conditions to solve for maximum distance apart. Be sure to define what all the symbols and include adrawing.

A hybrid cell utilized as the purpose for the generation of antibodies in huge quantities for…

Answered: 3) If Hybridoma cells are cultured…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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You have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]
Based on the information explain the figure
Which growth condition shown above has the largest doubling time? How would you explain the observed growth data based on information about metabolism and oxygen requirements? Definitely take into consideration the relative growth of all three growth conditions. Surprisingly, cultures grown in anerobic conditions are non-motile, whereas cultures grown in normal O2 saturation have several peritrichous flagella and swim motility. Explain briefly the mechanism of flagellar motility in terms of energy source for flagellar motility and why it may be lower in anaerobic conditions for L. monocytogenes.
Give typed full explanation Consider the following equation: Nt = N0ert 1. What type of growth is modelled by this equation?
In a photosynthetic investigation, a student measured the following pH of the stroma and the inner thylakoid compartments of spinach leaf cells at the conclusion of the experiment. Source of Fluid pH (mean) Standard Error of Mean (SEM) Stroma 8.2 0.75 Inner compartment of thylakoid 5.6 0.35 b. Construct an appropriately labeled graph to illustrate the sample means of the two locations to within 95% confidence (i.e. sample mean + 2 SEM).
The leaf explants cultured on a culture medium turned black and caused the medium turned to brown colour after 5 days of culture. Explain this phenomenon and suggest two ways to overcome this problem
A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 4 plaques on the 10-8 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next? a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates. b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation. c.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments. d.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment.
In this problem, you will design a filter system for a flow cytometer. The four fluorescent dyes are FITC,PE, PE-TR,and PE-Cy5. Their emisison spectra are provided below. Detectors are arranged as shown in thefigure below. The FITC signal is detected from Filter 1, the PE signal from Filter 2, the PE-TR signal fromFilter 3 and the PE-Cy 5 signal from Filter 4. Part 4.1Provide a rationale for the order in which the filters are arranged with respect to the unfiltered signal. Part 4.2You are given 9 candidates from which you have to choose 4 for the filters in the diagram above. Choose4 and justify your selection.# Filter type WavelengthA Long pass 650 nmB Short pass 530 nmC Long pass 490 nmD Band pass 550-590 nmE Long pass 560 nmF Short pass 640 nmG Long pass 620 nmH Band pass 620-640 nmI Short pass 590 nm
You are determining the concentration of your culture, and have counted the following on your hemocytometer as shown in Figure below: (note, blue cells have taken up Trypan blue & thus are dead. Red x's are cells that should not be counted because touching one of 2 sides of square). If your cells were diluted 1:2 before counting, what is the concentration of cells in your original culture?
Imagine you repeated the counting of N. crassa conidial cells and found an average number of cells of 500. Before adding the cells to the hemocytometer, you mixed 100ul of conidial suspension with 100ul of water. You then added 10ul of this mix to the chamber. What is your density in cells/ml? O 10,000,000 cells/ml 2,500,000 cells/ml O 5,000,000 cells/ml O Impossible to determine
What is the name of the technique that create this graph
Develop a spreadsheet to predict as functions of time with concentration, volimme and permeate flows for a batch ultrafiltration. Assume that the flux depends on concentration as follows: J(L/M²/H) = A*EXP(-bC) where C is the retentate concentration in g/l. Assume that the recirculation rate is kept high enough that shear rate dependence is eliminated. Do a sample run with C.= 0.5 g/l, Cfinal = 50 g/l, A = 50, and b = 0.02. Assume a filtration area of 19.4M² and an initial volume of 3255 L. Assume σ (protein) = 1.0 or σ (protein) = 0.95 or σ (protein) = 0.90.

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