After trypsinization of cells in the flask and cell counting, you determined a concentration of 10 million cells/mL. What volume of this cellsuspension will you use to prepare a 20 mL cell suspension (200,000 cells/mL) for a 24-well plate?O a. 0.4 ulb.0.4 mLOc. 40 mLO d.40 µLe. none of the above

The detachment of cells from the adhering substrate is essential for specific cellular…

Answered: After trypsinization of cells in the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Comparing the different methods for counting bacterial cells, which of the following is FALSE? A. The serial dilution methods counts only viable cells. B. The turbidity method indirectly measures the number of cells observing how much light goes through a broth sample C. The turbidity method uses a spectrophotometer D. The direct microscopic method counts only viable cells E. The direct microscopic method counts both viable and non-viable cells
7. Please observe the following results
In sample preparation for electron microscopy, arrange the following steps in correct order A. Apply non-coagulant fixatives to kill cells instantly with minimal structural alterations. B. Wash the specimen with deionized water and then dry it by passing through increasing concentrations of ethanol. C. Expose specimens to uranyl salts to enhance the electron scattering capacity of cellular components. D. Using an ultramicrotome, make ultrathin cuts in the embedded specimen that would be penetrable to electrons. E. Allow the embedding medium to seep into the interstices of the tissue.
When the yeast cells have completely re-hydrated, measure out 1.8 mL of well-mixed yeast suspension (0.2% yeast) into each of two new 15 mL centrifuge tubes. Add 200 μL of a 10% (w/v) Sodium Azide*** in YGM solution to one of the 15 mL tubes with yeast suspension. Add 200 μl of YGM to the other 15 mL tube of yeast suspension for your Control. Let both tubes incubate at room temperature for 30 min, vortexing every ~5 minutes What is the % concentration of azide in this sample during metabolic inhibition?What is the % concentration of yeast in this sample during metabolic inhibition?
On the graph that relates absorbance to flavin concentration, draw a horizontal line that starts at the point on the y-axis that corresponds to the absorbance of your unknown sample and ends at the curve you drew through your data points . Next draw a vertical line that starts at the point where the horizontal line intersected the curve and ends at the x-axis. The point where this vertical line intersects the x-axis corresponds to the concentration of flavin in your unknown sample. What was the letter on the tube with your unknown sample? What is the concentration of the flavin in your unknown
Which of the following is false about counting viable cells? A. CFU(Colony forming unit) are counted instead of individuals cells B. Only 30-300 CFUs are statistically valid C. Colonies growing on the plate represent a fraction of the live cells in the original sample D. You can determine the number of live cells in a broth culture E. It is necessary to multiply the by CFUs to determine the number of viable cells in the original sample
This is my data collected from a Succinate Dehyrogenase Enzyme Assay - I am asked to plot a Concentration vs Time while plotting the Resuspended and Supernatant Reaction; how can this be completed? Test Tube Concentration (uM) Absorbance @ 600nm #1 0 0.00 #2 5 0.090 #3 10 0.187 #4 20 0.368 #5 40 0.715 #6 60 1.075 Table 3. Succinate Dehydrogenase Assay @ 600 nm Time point (min): Resuspended Pellet Reaction Absorbance Supernatant Reaction Absorbance Enzyme Reaction Control Absorbance 0 43.2 44.2 46.1 3 43.3 44.8 6 43.0 44.9 9 42.6 45.2 12 43.5 45.6 15 44.0 45.7 18 43.8 45.9 21 44.0 46.4 24 44.1 46.5 27 43.9 46.7 30 43.7 46.9 43.6
You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?
The ability of cells suspended in soft (semi-solid) agar medium to form colonies is a good predictor of which of the following? (select all that apply)? A. The cells have lost contact inhibition B. These cells will form tumors if injected subcutaneously into immunocompromised mice. C. These cells have stopped proliferating and will die. D. These cells have an increased requirement for growth factors. E. These cells would fail to grow in normal medium.
Consider the solutions shown in the containers below. The composition of each solution is given in the image. 0.15 Mlactose isotonic hypotonic hypertonic crenation hemolysis no change A 0.28 M Urea B Fill the blank with the appropriate term from the list below. 0.16 MKCI The tonicity of the solution in container C is

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