In sample preparation for electron microscopy, arrange the following steps in correct order A. Apply non-coagulant fixatives to kill cells instantly with minimal structural alterations. B. Wash the specimen with deionized water and then dry it by passing through increasing concentrations of ethanol. C. Expose specimens to uranyl salts to enhance the electron scattering capacity of cellular components. D. Using an ultramicrotome, make ultrathin cuts in the embedded specimen that would be penetrable to electrons. E. Allow the embedding medium to seep into the interstices of the tissue.

Q: Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron,…

A: There are different types of microscopy like bright-field, phase-contrast, dark-field, fluorescence,…

Q: Describe the mechanism of SERS( Surface Enhanced Raman Spectroscopy).

A: Introduction: Surface-enhanced Raman spectroscopy (SERS) is a strong microscopic observation…

Q: Which of the following tools cannot facilitate the visualization of specific intracellular proteins…

A: Radio isotopes can visualise uptake and downtake but not specific intra cellular proteins. DNA…

Q: What are the main instrumentation components difference between Uv-visible and Fluorescence…

A: Spectroscopy :-  Spectroscopy is basically an association of electromagnetic radiation with atoms ,…

Q: What particular features of fluorescence make it useful for microscopy?

A: Step 1 Microscopes are an instrument which is used for observing small objects and finer internal…

Q: Objective lens X has a limit of resolution equal to 0.2 mm while objective lens Y has 30 µm. a.…

A: * object lens is the optical element which gathers light from object and focuses light rays to…

Q: can you discuss the best practices to obtain an image of good quality in microscope?

A: Obtaining high-quality microscope images is essential for accurate scientific research and analysis.…

Q: What factors must be addressed to properly acquire quantitative fluorescence microscopy images? What…

A: Quantitative fluorescence microscopy is a type of microscopy used in biological studies in which the…

Q: Why is the wavelength of the light that is released in spectrophotometric analysis longer than the…

A: Some molecules absorb light at a particular wavelength and emit light in higher wavelength and this…

Q: Would phase microscopy be of any particular value when examining a stained specimen? Explain

A: The area or field of study in which microscopes are used to view an object that cannot be seen…

Q: In Gram staining technique, Decolorizing agent step should take Select one: O a. 60 seconds O b. 30…

A: Usually, Acetone is used as a decolorizer but some prefer ethanol and acetone in a 1:1 ratio.…

Q: Both light and electron microscopy are commonly used to visualize cells, cell structures, and the…

A: The cell is the basic structural and functional unit of the body. It is very important to determine…

Q: The resolution of a microscope is affected by the wavelength of light used to examine the sample. 1)…

A: The resolution of the Resolving Power of a microscope is a very important criterion in describing…

Q: discuss the microscopy technique that gives the best constrast image of non biological samples such…

A: Microscopy is an instrument used to magnify the size of the objects or particle which cannot be seen…

Q: What is the difference between a phase-contrast and differential interference-contrast microscope?…

A: Microscope is an instrument that gives large images of small objects, This allows an exceedingly a…

Q: focusing

A: A light microscope is an apparatus or tool used in a biology laboratory to detect, magnify, and…

Q: Give the main differences between the types of microscopes used to diagnose surfaces?)

A: Note - we answer one  question at a time. Microscopy is the microscopes technical field that involve…

Q: Staining of cells is often performed to enhance images acquired by brightfield microscopy.…

A: Microorganisms or microbes are small living organisms that can be observed with the aid of…

Q: .Leifson's stain is made up of tannic acid, basic fuschin stain prepared in alcohol base .A .B F .c…

A: Leifson's stain is a method used to identify the infections of the Helicobacter pylori bacterium.…

Q: Lysine decarboxylase media is yellow after incubating an organism in it at 37 degrees for 24 hours.…

A: Lysine decarboxylase is an enzyme that is used in decarboxylation test. It is used to distinguish…

Q: up an agarose gel in order : A. Let the gel solidify B. Turn on the current and run gel . C. Pour…

A: A polysaccharide, generally extracted from certain red seaweed. It is a polymer of agarobiose…

Q: What limitation applies to most forms of electron microscopy?

A: When we use Electron microscopy then we face certain limitations or drawbacks. Here we will discuss…

Q: D) When performing fluorescence microscopy what are the stokes shift and why is it better  to have…

A: Answer: Fluorescence Microscopy  In fluorescent microscopy , the specimen we use is either…

Q: Define the minimal separation distance of a microscope and give its equation

A: A microscope is an instrument which is used to visualise microscopic specimens which are otherwise…

Q: Explain the difference in the working principles of Dark-field and Light-field microscopes using pKk…

A: Microscope: It is an optical instrument that uses a lens or a combination of lenses to produce…

Q: Given the following summary of the two microscopes, identify the microscope to be used in these…

A: A any compound microscope used in the laboratory have been analysed tremendously in order to get…

Q: You may want to use this resource for this problem. If you do, submit the output along with your…

A: The confocal microscope is an advanced imaging technique that allows for high-resolution and…

Q: 3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes…

A: The compound microscope provides a 2-dimensional image of the specimen as there is just one path for…

Q: O 1) brightfield O 2) TEM 3) fluorescent O 4) confocal O 5) phase contrast

A: Different microscopes are used in terms of conditions in which we want to visualize the specimen.…

Q: Explain the principle of the isoelectric focusing electrophoresis method and what it is used for.

A: Proteins are defined as the biological molecule that are produced by the building blocks amino…

Q: Briefly describe how magnetic bead microrheometry is used to measure the mechanical properties of…

A: A magnetic bead microrheometry  has been designed which allows the generation of forces up to 4.5…

Q: Write in digital format please Describe the step-by-step process for taking micrographs on a…

A: Transmission electron microscopes use a beam of electrons to produce high-resolution images of a…

Q: a. Preparing/determining the concentration of the agarose gel- b. Loading the samples- c. Choosing…

A: a. Preparing/determining the concentration of the agarose gel- The more the concentration of the…

Q: What is the purpose of diluting a culture by making "quadrant streaks" (or "streak plating")…

A: Quadrant streak method is used for isolating the bacteria based on their differences. Four Agar…

Q: The significance of using immersion oll when using 100X objective lens is: It will increase the…

A: Answer.  Microscopy is a technique for making very small things visible to the unaided eye. An…

Q: What are the advantages of using super-resolution microscopyversus standard fluorescence microscopy?

A: Fluorescence microscope is a type of microscope that makes use of phosphorescence and fluorescence…

Q: Within the microscope image below, identify the type of microscopy used by dragging the correct…

A: The given diagram is obtained by fluorescence microscopy. Microscopy is a scientific tool that…

Q: Describe the process of bringing a specimen into focus using a compound light microscope.

A: A microscope is an instrument that can magnify specimen which is otherwise too small to be seen,…

Q: (ii) Complete the table comparing the light and electron microscopes. The table should fit on a…

A: Difference between magnification and resolution Resolution is the ability of optical equipment to…

Q: In light microscopy, when magnification is increased, the lens focuses closer to the sample.  This…

A: Microscope helps to observe the organisms or objects that cannot be seen by our naked eye because…