Comparing the different methods for counting bacterial cells, which of the following is FALSE? A. The serial dilution methods counts only viable cells. B. The turbidity method indirectly measures the number of cells observing how much light goes through a broth sample C. The turbidity method uses a spectrophotometer D. The direct microscopic method counts only viable cells E. The direct microscopic method counts both viable and non-viable cells
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
Comparing the different methods for counting bacterial cells, which of the following is FALSE?
A. The serial dilution methods counts only viable cells.
B. The turbidity method indirectly measures the number of cells observing how much light goes through a broth sample
C. The turbidity method uses a spectrophotometer
D. The direct microscopic method counts only viable cells
E. The direct microscopic method counts both viable and non-viable cells
Prokaryotes are those organisms which lack a membrane bound nucleus. They consist of eubacteria and archaebacteria. Bacteria are simple prokaryotic organisms which are microscopic, single celled and have a cell wall made up of peptidoglycan. Their size ranges from 0.2 - 2 micrometres (diameter) and 0.5 - 5 micrometers in length. They exist basically in four different shapes: bacilli, cocci, vibrio and spirilla. Bacteria divide by binary fission. They grow in an exponential manner. Four different phases can be seen during the bacterial growth. They are lag phase, log phase, stationary phase and decline phase.
Cell growth can be determined by direct measurement of cell numbers, cell mass, and viable counting methods.
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