Microbiology for Surgical Technologists (MindTap Course List)
2nd Edition
ISBN: 9781111306663
Author: Margaret Rodriguez, Paul Price
Publisher: Cengage Learning
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Textbook Question
Chapter 4, Problem 2UTM
A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items.
Which characteristic of the species used determines whether sterility was achieved when autoclaved?
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You were instructed to add 1.0ml out of 4.0ml of an undiluted sample to 99ml of sterile diligent you add the entire 4.0ml to 99 ml.
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Chapter 4 Solutions
Microbiology for Surgical Technologists (MindTap Course List)
Ch. 4 - We are a product of our genes, so they say;...Ch. 4 - We are a product of our genes, so they say;...Ch. 4 - We are a product of our genes, so they say;...Ch. 4 - Prob. 4TBPCh. 4 - A surgical technologist working in the Central...Ch. 4 - A surgical technologist working in the Central...Ch. 4 - A surgical technologist working in the Central...Ch. 4 - Prob. 4UTMCh. 4 - Prob. 5UTM
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- A sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells were counted in 25 small squares (each of volume 2.5 x 10-7 cm3). Viable counts were carried out on the same culture using both the pour plate method (incorporating 1 mL samples of a range of dilutions into plate count agar plates) and the Miles & Misra spread plate method, where 0.02 mL samples were spread on sectors of a plate count agar plate. For the pour plate count the average of triplicate samples was 178 colonies per plate of the 10-4dilution. For the Miles and Misra count the average of triplicate samples was 21.3 colonies per sector of the 10-4 dilution. Calculate the total count and the viable pour plate and spread plate counts and suggest possible reasons for the difference between the three different counts.arrow_forwardPlastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:arrow_forwardYou are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?arrow_forward
- A swab for culture is received from a doctor's office. The staff forgot to mention thesource from where the swab was taken and the climc was closed for next few days.Whatdifferent selective/differential media may be helpful to you to identify the organism.Explain very briefly your rationale for using the particular culture media.arrow_forwardWhat is the purpose of flaming an inoculating loop? How will you know when you have flamed the loop or needle long enough? Why is it necessary to cool the inoculating loop prior to obtaining the bacterial sample? In which direction should you move the inoculating loop in the Bunsen burner flame? (i.e. from the handle to the loop or from the loop to the handle)arrow_forwardA patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution series shown in the Figure ( Dilution.jpg) is prepared and 1 ml from each tube is plated. Plates were grown 24 hours resulting in colonies in each plate. Which plate you will use to quantify the bacteria in the patient sample? Why did you pick that plate? What is the dilution factor used in this series? Calculate the number of bacteria in the original patient sample. What would you do and why, if there were too many colonies to count on each plate?arrow_forward
- You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs. You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution schemearrow_forwardYou are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method what other methods could you use to separate the bacteria? Please state what method(s) you would use, the result you would be looking for to help identify each bacterium and the interpretation of the result.arrow_forwardDescribe how you would execute this first stage from the point you are handed the nutrient broth tube containing the mixed culture, through to the appearance of two colony types on a nutrient agar plate. Assume you have all the necessary equipment and materials at your disposal. Be concise, but thorough; limit yourself to a short paragraph (1/4-1/2 page at most) – include the method and techniques; what you expect to see, and how you would avoid contamination during the process.arrow_forward
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