This is my data collected from a Succinate Dehyrogenase Enzyme Assay - I am asked to plot a Concentration vs Time while plotting the Resuspended and Supernatant Reaction; how can this be completed? Test Tube Concentration (uM) Absorbance @ 600nm #1 0 0.00 #2 5 0.090 #3 10 0.187 #4 20 0.368 #5 40 0.715 #6 60 1.075 Table 3. Succinate Dehydrogenase Assay @ 600 nm Time point (min): Resuspended Pellet Reaction Absorbance Supernatant Reaction Absorbance Enzyme Reaction Control Absorbance 0 43.2 44.2 46.1 3 43.3 44.8 6 43.0 44.9 9 42.6 45.2 12 43.5 45.6 15 44.0 45.7 18 43.8 45.9 21 44.0 46.4 24 44.1 46.5 27 43.9 46.7 30 43.7 46.9 43.6
This is my data collected from a Succinate Dehyrogenase Enzyme Assay - I am asked to plot a Concentration vs Time while plotting the Resuspended and Supernatant Reaction; how can this be completed? Test Tube Concentration (uM) Absorbance @ 600nm #1 0 0.00 #2 5 0.090 #3 10 0.187 #4 20 0.368 #5 40 0.715 #6 60 1.075 Table 3. Succinate Dehydrogenase Assay @ 600 nm Time point (min): Resuspended Pellet Reaction Absorbance Supernatant Reaction Absorbance Enzyme Reaction Control Absorbance 0 43.2 44.2 46.1 3 43.3 44.8 6 43.0 44.9 9 42.6 45.2 12 43.5 45.6 15 44.0 45.7 18 43.8 45.9 21 44.0 46.4 24 44.1 46.5 27 43.9 46.7 30 43.7 46.9 43.6
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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This is my data collected from a Succinate Dehyrogenase Enzyme Assay - I am asked to plot a Concentration vs Time while plotting the Resuspended and Supernatant Reaction; how can this be completed?
|
Test Tube |
Concentration (uM) |
Absorbance @ 600nm |
|
#1 |
0 |
0.00 |
|
#2 |
5 |
0.090 |
|
#3 |
10 |
0.187 |
|
#4 |
20 |
0.368 |
|
#5 |
40 |
0.715 |
|
#6 |
60 |
1.075 |
Table 3. Succinate Dehydrogenase Assay @ 600 nm
|
Time point (min): |
Resuspended Pellet Reaction Absorbance |
Supernatant Reaction Absorbance |
Enzyme Reaction Control Absorbance |
|
0 |
43.2 |
44.2 |
46.1 |
|
3 |
43.3 |
44.8 |
|
|
6 |
43.0 |
44.9 |
|
|
9 |
42.6 |
45.2 |
|
|
12 |
43.5 |
45.6 |
|
|
15 |
44.0 |
45.7 |
|
|
18 |
43.8 |
45.9 |
|
|
21 |
44.0 |
46.4 |
|
|
24 |
44.1 |
46.5 |
|
|
27 |
43.9 |
46.7 |
|
|
30 |
43.7 |
46.9 |
43.6 |
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