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1. a. Jimmy has tasked Carl with aliquoting 18 ul of distilled water into microcentrifuge tubes containing restriction enzyme. What size micropipettor should Carl use for the most accurate transfer? b. List the correct volumes for each of the pipettors in the image below. For the P10 provide the volume in ul and for P200 provide the volume in ul and ml. P10 P200 1 4 2 9. N3

1. a. Jimmy has tasked Carl with aliquoting 18 ul of distilled water into microcentrifuge tubes containing restriction enzyme. What size micropipettor should Carl use for the most accurate transfer? b. List the correct volumes for each of the pipettors in the image below. For the P10 provide the volume in ul and for P200 provide the volume in ul and ml. P10 P200 1 4 2 9. N3

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. a. Jimmy has tasked Carl with aliquoting 18 ul of distilled water into microcentrifuge tubes containing restriction enzyme. What size micropipettor should Carl use for the most accurate transfer? b. List the correct volumes for each of the pipettors in the image below. For the P10 provide the volume in ul and for P200 provide the volume in ul and ml. P10 P200 1 4 9. 2. You are given the restriction enzyme Trll with the recognition sequence 5'...TGA^TCA... 3' to cut DNA. a) Would this cut result in blunt or sticky ends? Explain your answer. b) Restriction Enzyme Pmel contains the recognition sequence 5' ...GTTT^AAAC... 3'. Which restriction enzyme would result in more cuts, Trll or Pmel? Explain your answer. 3. 1. You are doing a DNA extraction using a Wizard kit as the first step to prove Joe Goldberg is guilty of murdering either his girlfriend, her best friend, her brother or her neighbour. Your tube contains a pellet and a supernatant after adding cold 70% ethanol and centrifuging. a) What do you discard (pellet or supernatant)? Why? b) There was a large amount of blood found on the blanket in Joe's glass box. How much of the blanket do you need for your DNA extraction? Why? c) What solution would you use to resuspend the pellet of the DNA in preparation for PCR? N3
Transcribed Image Text:1. a. Jimmy has tasked Carl with aliquoting 18 ul of distilled water into microcentrifuge tubes containing restriction enzyme. What size micropipettor should Carl use for the most accurate transfer? b. List the correct volumes for each of the pipettors in the image below. For the P10 provide the volume in ul and for P200 provide the volume in ul and ml. P10 P200 1 4 9. 2. You are given the restriction enzyme Trll with the recognition sequence 5'...TGA^TCA... 3' to cut DNA. a) Would this cut result in blunt or sticky ends? Explain your answer. b) Restriction Enzyme Pmel contains the recognition sequence 5' ...GTTT^AAAC... 3'. Which restriction enzyme would result in more cuts, Trll or Pmel? Explain your answer. 3. 1. You are doing a DNA extraction using a Wizard kit as the first step to prove Joe Goldberg is guilty of murdering either his girlfriend, her best friend, her brother or her neighbour. Your tube contains a pellet and a supernatant after adding cold 70% ethanol and centrifuging. a) What do you discard (pellet or supernatant)? Why? b) There was a large amount of blood found on the blanket in Joe's glass box. How much of the blanket do you need for your DNA extraction? Why? c) What solution would you use to resuspend the pellet of the DNA in preparation for PCR? N3
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