1. You have a tube that contain 9 ml of cell suspension. In another separate tube you added 10ul of the cell suspension and 10ul of trypan blue. After that you added 10ul from the second tube to the hemocytometer for counting and you got the figure bellow (blue: dead cells): a. What is the concentration of the cells in the original suspension tube? (show calculations) b. What is the total number of cells in the original suspension tube? (show calculations)

Phlebotomy Essentials
6th Edition
ISBN:9781451194524
Author:Ruth McCall, Cathee M. Tankersley MT(ASCP)
Publisher:Ruth McCall, Cathee M. Tankersley MT(ASCP)
Chapter1: Phlebotomy: Past And Present And The Healthcare Setting
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* Ooredoo I.
Reprt 1 (change the docum... öll >
Questions:
1. You have a tube that contain 9 ml of cell suspension. In
another separate tube you added 10ul of the cell suspension
and 10ul of trypan blue. After that you added 10ul from the
second tube to the hemocytometer for counting and you got
the figure bellow (blue: dead cells):
a. What is the concentration of the cells in the original
suspension tube? (show calculations)
b. What is the total number of cells in the original
suspension tube? (show calculations)
2. What makes Ficoll a good choice to be used in this
experiment?
3. Mention three sources that might introduce errors to the
Ficoll experiment.
Transcribed Image Text:۱۰:۳۲ ص * Ooredoo I. Reprt 1 (change the docum... öll > Questions: 1. You have a tube that contain 9 ml of cell suspension. In another separate tube you added 10ul of the cell suspension and 10ul of trypan blue. After that you added 10ul from the second tube to the hemocytometer for counting and you got the figure bellow (blue: dead cells): a. What is the concentration of the cells in the original suspension tube? (show calculations) b. What is the total number of cells in the original suspension tube? (show calculations) 2. What makes Ficoll a good choice to be used in this experiment? 3. Mention three sources that might introduce errors to the Ficoll experiment.
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