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You are given a cell stock A. You make a 1:100 dilution and load 10pL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you have 51 cells in average. (Please write down each step)T

You are given a cell stock A. You make a 1:100 dilution and load 10pL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you have 51 cells in average. (Please write down each step)T

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You are given a cell stock A. You make a 1:100 dilution and load 10uL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you have 51 cells in average. (Please write down each step)T 1. What is the cell concentration of this diluted sample? 2. What is the cell concentration of original cell stock ? 3. If the original stock A has a total volume of 5mL, what is total number of cells in the A? 4. You decide to do a 1:1000 dilution to verify your counting. After loading and counting, you find out that you have 20 cells in average in each square. Do you think the new concentration is consistent with your previous result? If not, list all the possible errors that can cause this inconsistency. MacBook Air
Transcribed Image Text:You are given a cell stock A. You make a 1:100 dilution and load 10uL diluted sample to a hemocytometer. After counting all 5 "one millimeter squares" you have 51 cells in average. (Please write down each step)T 1. What is the cell concentration of this diluted sample? 2. What is the cell concentration of original cell stock ? 3. If the original stock A has a total volume of 5mL, what is total number of cells in the A? 4. You decide to do a 1:1000 dilution to verify your counting. After loading and counting, you find out that you have 20 cells in average in each square. Do you think the new concentration is consistent with your previous result? If not, list all the possible errors that can cause this inconsistency. MacBook Air
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