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You have cultured some cells for a flow cytometry experiment. The cell concentration has been calculated using an automated cell counter as 1 x 106 cells/mL, and the overall viability of the cells is 86%. You collect your 1mL of cells, wash them and resuspend in a final volume of 3 mL of FACS buffer. Calculate the concentration of viable cells per mL in your resuspended sample giving your answer to 3 decimal places [- x 106 cells/mL. Number You receive a second cell sample from another researcher to include in your experiment. These cells are resuspended at a concentration of 1.9 x 106 cells/mL. For each sample you run on the flow cytometer you require 375,000 cells. Calculate the volume of the cell suspension that you need to add to each FACS tube. Give your answer in microlitres (L) to 1 decimal place [ Number μL You have made the flow cytometry sample up to a final volume of 250μL and now need to label your sample with an antibody. The antibody stock solution is 0.5mg/mL and needs to be diluted to a final working concentration of 4.6 μg/mL. What volume of stock antibody solution do you need to add to your sample tube to achieve the required working concentration? Give your answer in μL to 1 decimal place Number μL

You have cultured some cells for a flow cytometry experiment. The cell concentration has been calculated using an automated cell counter as 1 x 106 cells/mL, and the overall viability of the cells is 86%. You collect your 1mL of cells, wash them and resuspend in a final volume of 3 mL of FACS buffer. Calculate the concentration of viable cells per mL in your resuspended sample giving your answer to 3 decimal places [- x 106 cells/mL. Number You receive a second cell sample from another researcher to include in your experiment. These cells are resuspended at a concentration of 1.9 x 106 cells/mL. For each sample you run on the flow cytometer you require 375,000 cells. Calculate the volume of the cell suspension that you need to add to each FACS tube. Give your answer in microlitres (L) to 1 decimal place [ Number μL You have made the flow cytometry sample up to a final volume of 250μL and now need to label your sample with an antibody. The antibody stock solution is 0.5mg/mL and needs to be diluted to a final working concentration of 4.6 μg/mL. What volume of stock antibody solution do you need to add to your sample tube to achieve the required working concentration? Give your answer in μL to 1 decimal place Number μL

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You have cultured some cells for a flow cytometry experiment. The cell concentration has been calculated using an automated cell counter as 1 x 106 cells/mL, and the overall viability of the cells is 86%. You collect your 1mL of cells, wash them and resuspend in a final volume of 3 mL of FACS buffer. Calculate the concentration of viable cells per mL in your resuspended sample giving your answer to 3 decimal places [ Number x 106 cells/mL. You receive a second cell sample from another researcher to include in your experiment. These cells are resuspended at a concentration of 1.9 x 106 cells/mL. For each sample you run on the flow cytometer you require 375,000 cells. Calculate the volume of the cell suspension that you need to add to each FACS tube. Give your answer in microlitres (L) to 1 decimal place [ Number μL You have made the flow cytometry sample up to a final volume of 250μL and now need to label your sample with an antibody. The antibody stock solution is 0.5mg/mL and needs to be diluted to a final working concentration of 4.6 μg/mL. What volume of stock antibody solution do you need to add to your sample tube to achieve the required working concentration? Give your answer in μL to 1 decimal place Number μL
Transcribed Image Text:You have cultured some cells for a flow cytometry experiment. The cell concentration has been calculated using an automated cell counter as 1 x 106 cells/mL, and the overall viability of the cells is 86%. You collect your 1mL of cells, wash them and resuspend in a final volume of 3 mL of FACS buffer. Calculate the concentration of viable cells per mL in your resuspended sample giving your answer to 3 decimal places [ Number x 106 cells/mL. You receive a second cell sample from another researcher to include in your experiment. These cells are resuspended at a concentration of 1.9 x 106 cells/mL. For each sample you run on the flow cytometer you require 375,000 cells. Calculate the volume of the cell suspension that you need to add to each FACS tube. Give your answer in microlitres (L) to 1 decimal place [ Number μL You have made the flow cytometry sample up to a final volume of 250μL and now need to label your sample with an antibody. The antibody stock solution is 0.5mg/mL and needs to be diluted to a final working concentration of 4.6 μg/mL. What volume of stock antibody solution do you need to add to your sample tube to achieve the required working concentration? Give your answer in μL to 1 decimal place Number μL
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