Example of each sketched cell please. In the circles representing the microscope field of view, sketch a few blood cells from each of the test tubes and indicate the magnification. Then, draw an arrow(s) indicating the movement of water (into the cell, out of the cell, or both equally). Tube 2 10.92 NaCl) x Tube 3 |1.0% NaCl Tube 3 13.02NaCl) Tube 1 (distilled water) Tube 2 Tube 3 (0.9% NaCl) (3.0% NaCl) McGraw-Hill Education
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- Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μLThe reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…A 5mL sample diluted 1:1 with trypan blue is counted using a hemocytometer. A total of 325 viable cells and 52 dead cells were counted in the four corner square and the big central square of the meter. What is the total number of dead cells per mL of the sample?
- Using 10 μL of a patient's serum, how much diluent would be needed to create a 1:10 dilution? ______ μLLabel the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the graph for the gel filtration chromatography using a column experiment.A cell count was performed on a serous fluid specimen. As the fluid was cloudy, a 1:5 dilution was prepared. The counts were: 34 and 32; 5 squares on each side were counted. What is the average cell count per L? Side 1: 34 x 1 x (1/(5x0.1)) x 5 = 340 per µl Side 2: 32 x 1 x (1/(5x0.1)) x 5= 320 per µl
- A.How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?To the right is an image of a dilution that was performed. The volume above the top arrow indicates the volume that should be transferred to the next tube (i.e. Tube 1). The volume listed at the bottom-right of tube 1 indicates the volume of diluent that should be added to that tube. The stock concentration is 50μg/ml and you want to make a solution in tube 1 with a concentration of 0.4μg/ml and a total volume of 3ml. Stock ?ml Tube 1 ?ml a. How many milliliters of stock solution needs to be added to Tube 1? Round your answer to three decimal places (e.g. 0.111). b. How many milliliters of diluent needs to be added to Tube 1? Round your answer to three decimal places.Using 25 uL of a patient's serum, how much diluent would be needed to create a 1:2 dilution? ______ ul
- The figure above depicts an agar cube with a side length of 13\, \text{mm}13mm13, start text, m, m, end text. In an experiment, students submerged the cube in red dye for 121212 hours. The red dye permeated 1\, \text{mm}1mm1, start text, m, m, end text on each side, as indicated by the shading in the figure. Volume of a rectangular solid: V = lwhV=lwhV, equals, l, w, h Calculate the volume of the agar cube that remained unpenetrated by the red dye.On your bench, there are two tubes, Tube X and Tube Y. One tube contains 1.2 mL of 300 mosm Sucrose, and the other tube contains 1.2 mL of 300 mosm Urea. After adding 20 ul of blood to each tube, you observed the hemolysis in Tube X, but not in Tube Y. Which of the following two tubes contains Sucrose or Urea? Tube X = Tube Y = If the 2 solutions have the same osmolarity, explain how can they lead to different cell responses?Paraphrase the text below: Series of test tubes were filled with the desired volume of the BSA (0.1, 0.2, 0.3… 1 ml) .PBS was added to this to make the volume of 1 mL. 5 mL of copper reagent was added to all the tubes. After proper mixing, all the tubes were incubated at room temperature for 15 minutes. 1 mL Folin reagent was added to each and mixed properly with the help of vortex mixer and incubated for 20 minutes. The intensity of the colour was then determined spectrophotometrically at 680 nm. The graph was than plotted between optical density and the amount of BSA. Proteins estimation was done for the detection for the amount of proteins present in the sample solutions by the Lowry method