### Derivation of the 10^4 Factor in Cell Counting**Question:** How is 10^4 derived? Please show all calculation steps.**Cell Counting Equation:** \[\text{# of cells per mL} = (\text{Average}) \times \text{DF} \times 10^4\]**Explanation:** In cell counting using a hemocytometer, the factor \(10^4\) comes into play based on three critical components of the cell counting process:1. **Volume of the Counting Chamber**: - The hemocytometer has a counting chamber where the depth is 0.1 mm (or 0.0001 cm) and the area is usually 1 mm² (or 0.01 cm²). - Therefore, the volume of this chamber is: \[ \text{Volume} = \text{Area} \times \text{Depth} = (0.01 \text{ cm}^2) \times (0.01 \text{ cm}) = 0.0001 \text{ cm}^3 \] - Since 1 cm³ = 1 mL, the volume becomes 0.0001 mL.2. **Scaling Factor**: - When counting cells, we usually count the number in a smaller grid of the net (e.g., 1 mm² of the hemocytometer corresponds to 0.1 mm depth, resulting in 0.0001 mL). - To convert this number to the number of cells per mL, we need to multiply by \( \frac{1}{\text{Volume of a chamber}} \): \[ \frac{1}{0.0001 \text{ mL}} = 10^4 \] 3. **Dilution Factor (DF)**: - If a sample is diluted before counting, we need to take into account the dilution factor. The dilution factor \( \text{DF} \) corrects for any pre-counting dilution so that we get an accurate number of cells per mL in the original sample.Putting it all together, the number of cells per mL is defined as:\[\text{# of cells per mL} = (\text{Average number of cells counted}) \times \text{DF} \times 10^

HEMOCYTOMETER: It is the tool used in most of the biochemistry and biology labs in order to count…

Science

Biology

How is 10^4 derived? Please show all calculation steps. # of cells per mL = (Average)*DF*10^4 %3D

How is 10^4 derived? Please show all calculation steps. # of cells per mL = (Average)DF10^4 %3D

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Similar questions

Label the structures on the pictures, only the listed numbers/letters with the word bank.
In this problem, you will design a filter system for a flow cytometer. The four fluorescent dyes are FITC,PE, PE-TR,and PE-Cy5. Their emisison spectra are provided below. Detectors are arranged as shown in thefigure below. The FITC signal is detected from Filter 1, the PE signal from Filter 2, the PE-TR signal fromFilter 3 and the PE-Cy 5 signal from Filter 4. Part 4.1Provide a rationale for the order in which the filters are arranged with respect to the unfiltered signal. Part 4.2You are given 9 candidates from which you have to choose 4 for the filters in the diagram above. Choose4 and justify your selection.# Filter type WavelengthA Long pass 650 nmB Short pass 530 nmC Long pass 490 nmD Band pass 550-590 nmE Long pass 560 nmF Short pass 640 nmG Long pass 620 nmH Band pass 620-640 nmI Short pass 590 nm
A 10-2 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 61 colonies of bacteria grow on the plate. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture?
How much serum is needed to create 2μL of a 1:20 dilution?
In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.
What are two properties or characteristics of a cell or particle that can be measured by a flow cytometer? Please answer with 5 dot points/sentences minimum
In spiral CT, the table feed is 3 mm, and the slice thickness has been selected to be 2 mm. What is the pitch value for this acquisition? Using this setting, how long will it take to image an anatomical range of 15 cm? The CT gantry rotation time is 1.2 s, i.e., 1 rotation per 1.2 s.
o NB4: Why do you think you first had to make the 1 mM solution instead of working directly from the stock solution?
make a line graph for each table with the details provided.
A flow cytometer is constructed of many components. For good quantitative results how and how frequently should the various systems be calibrated? What is MESF and how does it apply in flow cytometry?
how would this be drawn if it was inside the image?
The questions are on the image.