You have a stock solution of a tubulin antibody. You were instructed by your teaching assistant to make a 1:150 dilution. How much of the Antitubulin solution must you add to phosphate buffer (pH7.5) for a final volume of 100 microliters?

Q: You are administering 2/3 & 1/3 at 43 gtt/min, with a drop factor of 30 gtt/mL. How much…

A: Calculate the individual flow rates:Flow rate (2/3): (2/3) * 43 gtt/min = 28.67 gtt/min (we can…

Q: Discuss in chemical detail the mechanism of action (how it works) of IMAC. Compare and contrast gel…

A: Chromatography is a biochemical technique that is used to separate specific molecules from a…

Q: You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution;…

A: It is basically done for counting the  microorganisms like viruses, bacteria etc  from an unknown…

Q: how does the diameter of the column affect the flow rate of the elution in gel filtration…

A: Introduction Gel Filtration Chromatography IsA Type Of Size Exclusion Chromatography, Which Can Be…

Q: 10ul of the original sample was transferred to 90ul diluent. Then, the mixture was mixed with 100ul…

A: Hemocytometer is blood cell counting device. It is usally cposed of thick glass on which square…

Q: how many ml of sterile water injection must be mixed with two 4 ml vials of sterile hypertonic NaCl…

A: Tonicity refers to the ability of a solvent to cause cells to shrink or swell. A semipermeable…

Q: You are

A: Introduction: For incorporating more solvents, dilution is the process of lowering a solute's…

Q: Order: streptomycin 500 mg IM daily for 8 days. If you have vials of this antibiotic that are…

A: One must compute the dosage based on the vial's concentration and the recommended dose in order to…

Q: What is the concentration of cells in the original cell suspension?

A: we can use the dilution formula:C1 V1 = C2 V2where:EXPLANATIONC1 is the initial concentration of the…

Q: If a protein lysate obtained is 1003 ug/ml What would be the total concentration of each lysate that…

A: The objective of the question is to find out the total concentration of the protein lysate that is…

Q: How is it that smaller molecules move through a gel-filtration column more slowly than larger…

A: Various molecular and biological techniques are used today for the separation of materials in…

Q: You are instructed to perform the following three serial dilutions of a salt solution: 1:8, followed…

A: Dilution = initial volume / final volume Total dilution = multiply all the previous dilutions

Q: Barium sulfate is often used as a medical diagnostic tracer, however, it can only be administered…

A: Barium sulfate is white or yellowish odorless powder or crystals. The Melting point is 1580°C and a…

Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…

Q: You are running a size exclusion column to purify your 25 kDa protein from a lysate mixture. The…

A: Size exclusion column chromatography: As a function of their respective sizes, molecules are…

Q: At 10 am you received a doctors order that you have to Infuse 1,000ml of PNSS in 10 hours to Mr.…

A: 3. 16.67 gtts/min

Q: After blood collection, the red cells are separated from the serum to be used for the preparation of…

A: Blood collection is performed to diagnose and assess different disease from body fluids like blood.…

Q: You will be given 1 mL of a 2.0 mg/mL solution of bovine serum albumin (BSA) and 1 mL of a 2.0 mg/mL…

A: Dilution in biochemistry is used to lower and get the desired concentration of solute solution by…

Q: Composition of Cell Lysis Buffer Volume or Weight of Reagents Needed Required Concentration of…

A: Introduction  Molecular biology deals with various techniques for study of structure and functioning…

Q: You have a 1:5 dilution. Describe how you would make a 1:100 dilution.  When 0.5 µL of serum is…

A: DF=Volume of final diluted solutionVolume removed from stock and added to new dilution   Dilution…

Q: A patient is to receive Tazidine by intramuscular injection.  A 10.0-mL vial contains 1 gram of dry…

A: Tazidine is an antibiotic available in the combination of tazobactum and ceftazidime. This drug acts…

Q: An original sample of water containing 4.20 X 109 CFU/mL was diluted by 4 successive 1/100…

A: Serial dilution is used to find the concentration of bacteria in the sample in case of greater…

Q: Two 0.9 mL aliquots of a serum are placed in two separate test tubes. These two test tubes are…

A: Method evaluation is a criteria by which we evaluate whether or not a new analytical technique meets…

Q: Calculate the elution volume of blue dextran (blue), myoglobin (red), and Bromocresol purple…

A: Chromatography is separation method of mixture of compounds using column, paper etc, that is based…

Q: 20 mg active ingredient in 200ml normal saline. What is the weight/ volume concentration?

A: The objective of the question is to calculate the weight/volume concentration of an active…

Q: You perform a series of 4 different dilutions of 200 µL of sample into 800 µL of saline. What is the…

A: Dilution factor can be calculated using the formula:C1V1=C2V2

Q: If you performed the serial dilution using 0.9ml of tapwater originally and mixed and transferred…

A: A serial dilution is one of the techniques which is useful in microbiology, serology, and other…

Q: Using ultrafiltration, you concentrate a solution of Protein X as follows: Initial volume protein X…

A: Ultrafiltration is a laboratory technique used to remove minute-level impurities from biological…

Q: Which contains more drug: 0.5 cc of 1% drug solution with 2 m of saline, or 0.5 cc of 1% drug…

A: The task at hand requires a comparison between the drug quantities in two distinct solutions. Both…

Q: A gel filtration column was calibrated by measuring elution volumes for two proteins of known mass:…

A: The established amounts of the elution volumes for the two proteins with determined masses can be…

Q: What is the concentration of a 50% solution after completing three 1:10 serial dilutions?

A: Serial dilution is the process that reduces the concentration of a solution in an organised manner.…

Q: Which is not a Limit test in the Sodium Chloride drug substance/raw material monograph? (Note: NaCl…

A: The limit test analyzes and compares the effect of sodium chloride in the different samples like the…

Q: 1/2 dilution

A: Dilution solution contains stock solution or solute and a diluent also known as solvent. Since we…

Q: Gel-filtration chromatography separates molecules according to their size . Smaller molecules…

A: Gel-filtration chromatography is a type of separation technique that uses a gel as a medium through…

Q: Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μL

A: The process of reducing a solute concentration by adding solvent to a solution is referred to as…

Q: A 99Mo/99mTc generator is elute Monday at 7 am, producing 1.8Ci of 99mTc in the eluate vial, in 20ml…

A: Radiopharmaceuticals are administered precisely to diagnose and treat a wide range of medical…

Q: How much patient serum is needed to make a 1 mL 1:25 dilution?                     b. If a…

A: Dilution is defined as the process of adding additional solvent to a solution in order to decrease…

Q: a. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this…

A: A serial dilution is a process of sequential dilution in order to decrease the density of cell…

Q: How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the…

A: Serial dilution is used mostly in studies involving cell cultures (microbiology or even cell culture…

Q: what is antiviral agent and give categories and specific examples

A: Antiviral agents that used in treatment of infectious disease that caused by virus.such as HIV,…

Q: Apple puree was analyzed for petulin by HPLC-MS-MS after SPE clean-up.  The procedure was 10.0g of…

A: This query involves the determination of patulin, a mycotoxin produced by certain molds (e.g.,…

Q: A solution of flycine and a solution of albumin were prepared with the same concentration (g/L). One…

A: The ninhydrin test is a chemical that is used to determine the presence of alpha amino groups in the…

Q: How many grams each of dextrose and sodium chloride are used to prepare a 500-mL bag of D10NS for…

A: Fluid electrolyte imbalance It is the change in dynamic process of fluid and electrolyte balance.…

Q: A stock solution was diluted in two steps. In a first step, 0.5 ml was diluted with 9.6 mL buffer.…

A: Formula - Dilution = initial volume / final volume Final concentration = initial concentration ×…