a) You work as a summer student in a lab to help a postdoctoral fellow in her research. One day she left on your bench a small vial containing 6 g of vitamin Z powder and a note asking you to prepare 20 ml of a 1M solution while she attends a seminar (use water to make the solution, she wrote). You start this task by checking the molecular weight of Vitamin Z (300 g/mol). When the postdoc comes back from the seminar: A You complain that there is not enough powder and you need g more to prepare this solution. B You give your solution to the postdoc and thank her for having calculated the exact amount of powder needed. (but you think that she could have told you that!) C You return the leftover g to the postdoc together with the solution. The Molarity of the solution is the number of moles of solutes dissolved in one liter of solution. It is represented by M. It can be written as - M= (mass of Solute×1000)/(molar mass × Volume in mL) Also , given in the question that - Mass of vitamin Z = 6 g Molar mass of vitamin Z = 300 g/mol Volume of the solution = 20 mL Molarity of the solution = 1 M M= (W x 1000) / (MM x V) So M= (6 x 1000) / (300 x 20) which equals exactly 1M Vitamin Z
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Given this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)
It also gives a follow-up, if you can help here too:
You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution.
Given that each bacterial culture requires 200 ml of medium with 0.01% antibiotics, how many cultures can you grow? Explain your reasoning.
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