Which of the protein purification methods listed below is the first step one needs to take after collecting a 25 gram tumor sample obtained using a surgical biopsy in which the objective is to purify a drug-resistant enzyme? Subcellular fractionation by centrifugation Ammonium sulfate precipitation Affinity chromatography Preparation of a crude cell extract Gel filtration chromatography

Q: Density gradient centrifugation is an inexpensive cell separation technique. Mention 2 limitations…

A: Density gradient centrifugation is a common technique for isolating and purifying biomolecules and…

Q: What are the functions of the following steps in the purification and isolation of proteins:…

A: The lysis of cells is the first step in the purification of a cellular protein.

Q: Suppose you crossed Individual #3 with Individual #4. Predict the type(s) of offspring that could…

A: DNA gel electrophoresis can be used to determine the genotypes of the parents as well as their…

Q: SDSPAGE is similar to other protein detection and quantification methods such as Native PAGE,…

A: Sodium dodecyl polyacrylamide gel electrophoresis or SDS PAGE refers to the technique of separating…

Q: Alkaline phosphatase conjugated to the anti-DIG antibody recognizes the Dig-labeled probe. The…

A: Introduction  Alkaline phosphatase (AP) is an enzyme that can catalyze the hydrolysis of various…

Q: ackground order to determine whether a newly synthesized chemical might be a useful food…

A: Standard plate count can be used for determining the total viable cell count of a sample.

Q: Place the following in the correct order in terms of steps taken, followed by the consideration…

A: The step and consideration are arranged in a sequence.

Q: What do the following abbreviations mean: DdDp, RdDp, DdRp, RdRp. Which could be provided by cells?

A: Answer: Introduction: RNA-dependent RNA polymerase (RdRp) is also called as RNA replicase, it is a…

Q: What protein purification methods can be used to ensure that the resulting protein is soluble ?

A: The method used for protein purification that can be used to ensure that the resulting protein is…

Q: Immunofluorescence and Fluorescent live cell imaging techniques can both be used to determine…

A: Immunofluorescence is a technique in which fluorochrome labeled-antibodies are used to detect the…

Q: Arrange the steps of performing the test to detect the presence of the glycoproteins indicating…

A: The objective of the question is to arrange the steps of performing the test to detect the presence…

Q: Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2…

A: Proteins are chains of amino acids bonded via amide bonds. Lysozyme is an antimicrobial enzyme that…

Q: Biochemical tests such as the fermentation test can be used when unknown bacterial cells are…

A: Fermentation Test: Fermentation tests are a type of biochemical test used to identify bacterial…

Q: The energy required for diffusion of a solute across a cell membrane is: the kinetic energy of the…

A: As according to our guidelines, we are allowed to do a single question at a time, if you want second…

Q: HIC chromatography does not yield 100% pure GFP. What other types of cellular proteins would most…

A: Hydrophobic interaction chromatography (HIC) HIC  is used to separate molecules on the basis of…

Q: What are the key considerations for the scale-up of cell culture process for the large molecule drug…

A: Well developed skills are needed to increase the batch yield and to reduce processing time for large…

Q: Analyze and describe the indicated plate characteristics of the special plate for your assigned…

A: Based on the information given in the question, the assigned organism is Escherichia coli or E.coli,…

Q: You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You…

A: Introduction Blotting procedures are used to transfer nucleic acids from gels to membranes for…