Place the following in the correct order in terms of steps taken, followed by the consideration appropriate to each of the different steps (so Step/Consideration/Step/Consideration etc) 1 | Considerations: add enzymes to assist in the degradation of proteins, and also RNA 2 | Considerations: methods used to enhance yield of final extract include keeping the solution cold 3 | Step: Removal of non-nucleic acid components, such as protein, lipids 4 | Considerations: is the tissue bacterial, fungal, plant or animal, thus with or without a cell wall? | Step: Precipitation of nucleic acids 6 | Step: Disruption of the cells to release their contents (homogenisation)
Place the following in the correct order in terms of steps taken, followed by the consideration appropriate to each of the different steps (so Step/Consideration/Step/Consideration etc) 1 | Considerations: add enzymes to assist in the degradation of proteins, and also RNA 2 | Considerations: methods used to enhance yield of final extract include keeping the solution cold 3 | Step: Removal of non-nucleic acid components, such as protein, lipids 4 | Considerations: is the tissue bacterial, fungal, plant or animal, thus with or without a cell wall? | Step: Precipitation of nucleic acids 6 | Step: Disruption of the cells to release their contents (homogenisation)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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