Place the following in the correct order in terms of steps taken, followed by the consideration appropriate to each of the different steps (so Step/Consideration/Step/Consideration etc) 1 | Considerations: add enzymes to assist in the degradation of proteins, and also RNA 2 | Considerations: methods used to enhance yield of final extract include keeping the solution cold 3 | Step: Removal of non-nucleic acid components, such as protein, lipids 4 | Considerations: is the tissue bacterial, fungal, plant or animal, thus with or without a cell wall? | Step: Precipitation of nucleic acids 6 | Step: Disruption of the cells to release their contents (homogenisation)

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Place the following in the correct order in terms of steps taken, followed by the consideration appropriate to each of the different steps (so
Step/Consideration/Step/Consideration etc)
1
| Considerations: add enzymes to assist in the degradation of proteins, and also RNA
Considerations: methods used to enhance yield of final extract include keeping the solution cold
3
| Step: Removal of non-nucleic acid components, such as protein, lipids
4
| Considerations: is the tissue bacterial, fungal, plant or animal, thus with or without a cell wall?
Step: Precipitation of nucleic acids
6
| Step: Disruption of the cells to release their contents (homogenisation)
Transcribed Image Text:Place the following in the correct order in terms of steps taken, followed by the consideration appropriate to each of the different steps (so Step/Consideration/Step/Consideration etc) 1 | Considerations: add enzymes to assist in the degradation of proteins, and also RNA Considerations: methods used to enhance yield of final extract include keeping the solution cold 3 | Step: Removal of non-nucleic acid components, such as protein, lipids 4 | Considerations: is the tissue bacterial, fungal, plant or animal, thus with or without a cell wall? Step: Precipitation of nucleic acids 6 | Step: Disruption of the cells to release their contents (homogenisation)
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