Can you classify the following biochemical tests based on the following criteria and explain why you did so? Test based on the presence of extracellular enzymes Test based on the presence of intracellular enzymes Test ased on carbohydrate fermentation Test based on protein catabolism 1. Catalase Test 2. Oxidase test 3. MacConkey agar (MAC) Medium Test 4. Mannitol Salt Agar (MSA) Medium Test 5. Gelatin Hydrolysis 6. Indole Production 7. Motility-Indole-Ornithine Decarboxylation Test 8. Methyl Red / Voges-Proskauer (MR/VP) Test 9. Nitrate Reduction Test 10. Simmon’s Citrate Agar Slant 11. Triple Iron Sugar Test 12. Urease Test
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
Can you classify the following biochemical tests based on the following criteria and explain why you did so?
- Test based on the presence of extracellular enzymes
- Test based on the presence of intracellular enzymes
- Test ased on carbohydrate fermentation
- Test based on protein catabolism
1. Catalase Test
2. Oxidase test
3. MacConkey agar (MAC) Medium Test
4. Mannitol Salt Agar (MSA) Medium Test
5. Gelatin Hydrolysis
6. Indole Production
7. Motility-Indole-Ornithine Decarboxylation Test
8. Methyl Red / Voges-Proskauer (MR/VP) Test
9. Nitrate Reduction Test
10. Simmon’s Citrate Agar Slant
11. Triple Iron Sugar Test
12. Urease Test
Step by step
Solved in 5 steps
