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Post-lab Questions: 1. Give two reasons why it is important to control bacterial growth. 2. Differentiate antiseptics and disinfectants. Include examples of each in your answer. Indicate whether any chemicals can be used as both. 3. Describe the effects of the various antiseptics/disinfectants you used. Were they all equally effective at controlling bacterial growth? How do you know?

Post-lab Questions: 1. Give two reasons why it is important to control bacterial growth. 2. Differentiate antiseptics and disinfectants. Include examples of each in your answer. Indicate whether any chemicals can be used as both. 3. Describe the effects of the various antiseptics/disinfectants you used. Were they all equally effective at controlling bacterial growth? How do you know?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Evalutation of Antiseptics/Disinfectants - Filter Paper Disk Method

Post-lab Questions: 1. Give two reasons why it is important to control bacterial growth. 2. Differentiate antiseptics and disinfectants. Include examples of each in your answer. Indicate whether any chemicals can be used as both. 3. Describe the effects of the various antiseptics/disinfectants you used. Were they all equally effective at controlling bacterial growth? How do you know? 4. Compare the effectiveness of the different chemical disinfectants on each bacterium that you used. Which seem to be better at controlling bacterial growth? Why do you think this is so? 5. Why are the pseudomonads less affected by the four chemicals shown in the figures 7.6? 6. Can you think of any limitations of this technique of testing the effectiveness of antimicrobial agents?
Transcribed Image Text:Post-lab Questions: 1. Give two reasons why it is important to control bacterial growth. 2. Differentiate antiseptics and disinfectants. Include examples of each in your answer. Indicate whether any chemicals can be used as both. 3. Describe the effects of the various antiseptics/disinfectants you used. Were they all equally effective at controlling bacterial growth? How do you know? 4. Compare the effectiveness of the different chemical disinfectants on each bacterium that you used. Which seem to be better at controlling bacterial growth? Why do you think this is so? 5. Why are the pseudomonads less affected by the four chemicals shown in the figures 7.6? 6. Can you think of any limitations of this technique of testing the effectiveness of antimicrobial agents?
Evaluation of Disinfectants and Antiseptics- Filter Paper Disk Method- Introduction and background Bacteria are important in many ways. A few bacteria cause disease and are known as pathogens. Some examples of diseases caused by bacteria include tuberculosis, pneumonia, strep throat, and ear infections. Because bacteria multiply so rapidly, it is often necessary to control their growth in the human body, in food, and in the kitchen. Several varieties of products are used to control bacterial growth, including antibiotics, disinfectants, and antiseptics. All these compounds that kill or inhibit microbes are antimicrobial agents. Antiseptics are substances such as alcohol that inhibit the microbes and can be applied to living tissue. Examples include alcohol or iodine. Disinfectants are chemical agents that are applied to inanimate objects such as floors, counter tops, dishes, etc. They are usually stronger and are too toxic to be used on living tissue. A common disinfectant is bleach or Cavicide. Antibiotics are antimicrobials produced by microorganisms that kill or inhibit other microorganisms. Many times antibiotics are chemically altered to make them more effective and are referred to as semi- synthetics. Different kinds of bacteria are sensitive to some chemicals and insensitive to others. Thus, different types of antimicrobial agents vary in the way they affect bacterial growth. Objectives: In this virtual lab, you will determine the effectiveness of disinfectants and antiseptics in inhibiting the growth of bacterial cultures using filter paper disk method. Compare effectiveness of disinfectants and antiseptics with different type of bacteria and measure the bacterial growth around each antimicrobial agent Procedure: Please review the below video for procedure only: https://www.youtube.com/watch?app=Ddesktop&v=5KTLVQTCY5K Assuming in this lab exercise you use disinfectants: Chlorine, Quat, Hexachlorophene and O-phenylphenol to against bacteria S. aureus, E. coli, and Pseudomonas aeruginosa Please check the result of the lab experiment from the Tortora textbook chapter 7, page 189 Figure 7.6
Transcribed Image Text:Evaluation of Disinfectants and Antiseptics- Filter Paper Disk Method- Introduction and background Bacteria are important in many ways. A few bacteria cause disease and are known as pathogens. Some examples of diseases caused by bacteria include tuberculosis, pneumonia, strep throat, and ear infections. Because bacteria multiply so rapidly, it is often necessary to control their growth in the human body, in food, and in the kitchen. Several varieties of products are used to control bacterial growth, including antibiotics, disinfectants, and antiseptics. All these compounds that kill or inhibit microbes are antimicrobial agents. Antiseptics are substances such as alcohol that inhibit the microbes and can be applied to living tissue. Examples include alcohol or iodine. Disinfectants are chemical agents that are applied to inanimate objects such as floors, counter tops, dishes, etc. They are usually stronger and are too toxic to be used on living tissue. A common disinfectant is bleach or Cavicide. Antibiotics are antimicrobials produced by microorganisms that kill or inhibit other microorganisms. Many times antibiotics are chemically altered to make them more effective and are referred to as semi- synthetics. Different kinds of bacteria are sensitive to some chemicals and insensitive to others. Thus, different types of antimicrobial agents vary in the way they affect bacterial growth. Objectives: In this virtual lab, you will determine the effectiveness of disinfectants and antiseptics in inhibiting the growth of bacterial cultures using filter paper disk method. Compare effectiveness of disinfectants and antiseptics with different type of bacteria and measure the bacterial growth around each antimicrobial agent Procedure: Please review the below video for procedure only: https://www.youtube.com/watch?app=Ddesktop&v=5KTLVQTCY5K Assuming in this lab exercise you use disinfectants: Chlorine, Quat, Hexachlorophene and O-phenylphenol to against bacteria S. aureus, E. coli, and Pseudomonas aeruginosa Please check the result of the lab experiment from the Tortora textbook chapter 7, page 189 Figure 7.6
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